Differential long noncoding RNAs expression in cancer-associated fibroblasts of non-small-cell lung cancer
Differential long noncoding RNAs expression in cancer-associated fibroblasts of non-small-cell lung cancer
复制标题
非小细胞肺癌癌症相关成纤维细胞中长非编码RNA的差异表达
DOI：
10.2217/pgs-2018-0102
复制
发表时间：
2019-02-01
期刊：
影响因子：
2.1
通讯作者：
Yang, Jie
中科院分区：
文献类型：
--
作者：
Teng, Cuifang;Huang, Guolin;Yang, Jie
目的：本研究的目的是探讨长链非编码RNA（lncRNA）在非小细胞肺癌（NSCLC）进展过程中癌相关成纤维细胞（CAF）中的作用。材料和方法：通过lncRNA微阵列检测三个患者配对的CAF和相邻的正常成纤维细胞之间差异表达的lncRNA和mRNA，所述成纤维细胞从三个原发性NSCLC手术切除的肺组织的肿瘤和非肿瘤部分获得。对这些差异表达的mRNA进行生物信息学分析，包括基因本体和通路分析。进行qRT-PCR以鉴定可能参与CAF对NSCLC的贡献的选定lncRNA的变化。结果：CAFs中共有766条lncRNA和750条mRNA异常表达（倍数变化>2，p
Aim: The aim of this study was to investigate the role of long noncoding RNAs (lncRNAs) profiles in cancer-associated fibroblasts (CAFs) during non-small-cell lung cancer (NSCLC) progression. Materials & methods: Differentially expressed lncRNAs and mRNAs were detected by lncRNA microarray between three patient-paired CAFs and the adjacent normal fibroblasts, which were obtained from tumoral and nontumoral portions of surgically resected lung tissue from three primary NSCLCs. Bioinformatic analyses including gene ontology and pathway analysis were applied to these differentially expressed mRNAs. The qRT-PCR was conducted to identify the change of selected lncRNAs that might be involved in contribution of CAFs toward NSCLC. Results:A total of766 lncRNAs and 750 mRNAs abnormally expressed in CAFs (fold-change >2, p