Functional differentiation of multiple climbing fiber inputs during synapse elimination in the developing cerebellum
Functional differentiation of multiple climbing fiber inputs during synapse elimination in the developing cerebellum
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DOI：
10.1016/s0896-6273(03)00298-8
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发表时间：
2003-06-05
期刊：
影响因子：
16.2
通讯作者：
Kano, M
中科院分区：
文献类型：
--
作者：
Hashimoto, K;Kano, M
我们研究了小脑攀升纤维(CF)到浦肯野细胞(PC)突触在发育过程中的生理特性是如何变化的。从P3到P6，多个CFS的强度差异变大。在P10左右，每个PC被一个强CF(CF-mono)单独支配，或由一个强CF(CF-MULTI-S)和几个较弱的CF(CF-MULTI-W)复合支配。我们发现，在多胎S、单声道和成熟期CFs中，每个部位同时释放多个囊泡的现象正常发生，而在多胎CFs和新生儿CFs中则较少发生。我们还提出了证据，表明具有较低多囊释放概率的较弱的CFS将优先被撤除。结果表明，在发育中的CFs突触消失之前，分化成具有高和低多囊释放概率的强和弱CFs。
We studied how physiological properties of cerebellar climbing fiber (CF) to Purkinje cell (PC) synapses change during developmental transition from multiple to mono CF innervation onto each PC. From P3 to P6, differences in the strengths of multiple CFs became larger. Around P10, each PC was either monoinnervated by one strong CF (CF-mono) or multiply innervated by one strong CF (CF-multi-S) plus a few weaker CFs (CF-multi-W). We show that simultaneous release of multiple vesicles per site occurs normally from CF-multi-S, CF-mono, and mature CFs, but less frequently from CF-multi-W and neonatal CFs. We also present evidence suggesting that weaker CFs with lower probability of multivesicular release would be withdrawn preferentially. The results suggest that differentiation into strong and weak CFs with high and low probabilities of multivesicular release precedes developmental CF synapse elimination.