Leptin effect on RANKL and OPG expression in MCM-E1 osteoblasts
Leptin effect on RANKL and OPG expression in MCM-E1 osteoblasts
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DOI：
10.1002/jcb.20853
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发表时间：
2006-08-01
影响因子：
4
通讯作者：
Barbosa, M. A.
中科院分区：
文献类型：
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作者：
Lamghari, M.;Tavares, L.;Barbosa, M. A.
最近的研究表明，瘦素激素可能通过直接调节OPG/RANKL平衡而在骨重建中发挥关键作用。本研究采用RT-PCR和ELISA方法研究瘦素对MC 3 T3-E1成骨细胞RANKL和OPG表达的影响。我们首先鉴定了MC 3 T3-E1中Ob-Rb和Ob-Ra瘦素受体亚型的表达，并观察到这些细胞对mrleptin处理有反应。然后我们研究了mrleptin对RANKL和OPG表达的影响。我们发现，mrleptin剂量依赖性地调节RANKL mRNA的表达，在浓度高于12 ng/ml时观察到完全抑制。通过sRANKL蛋白测量证实了该效应。然而，暴露于mrleptin对MC 3 T3-E1的OPG mRNA没有影响。综上所述，这些结果表明，瘦素通过抑制RANKL基因的表达来正向调节OPG/RANKL平衡。
Recent studies have suggested that leptin hormone may play a pivotal role on bone remodeling through a direct effect by modulating positively the OPG/RANKL balance. Here, we investigate the effect of leptin hormone on RANKL and OPG expression in MC3T3-E1 osteoblasts using RT-PCR and ELISA measurements. We have at first identified the expression of Ob-Rb and Ob-Ra leptin receptor isoforms in MC3T3-E1 and observed that these cells respond to mrleptin treatments. We then investigated the effect of mrleptin on RANKL and OPG expression. We show that mrleptin dose-dependently regulated the expression of RANKL mRNA with complete inhibition observed at concentrations higher than 12 ng/ml. This effect was confirmed with sRANKL protein measurements. However, the exposure of MC3T3-E1 to mrleptin had no effect on OPG mRNA. Taken together, these results suggest that leptin modulates positively OPG/RANKL balance by inhibiting the expression of RANKL gene.