ALTERNATIVE FORMS OF MAX AS ENHANCERS OR SUPPRESSORS OF MYC-RAS COTRANSFORMATION
ALTERNATIVE FORMS OF MAX AS ENHANCERS OR SUPPRESSORS OF MYC-RAS COTRANSFORMATION
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DOI：
10.1126/science.256.5055.373
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发表时间：
1992-04-17
期刊：
影响因子：
56.9
通讯作者：
ALITALO, K
中科院分区：
文献类型：
--
作者：
MAKELA, TP;KOSKINEN, PJ;ALITALO, K
Max是一种碱性-螺旋-环-螺旋-亮氨酸拉链蛋白，能够与Myc蛋白形成序列特异性DNA结合复合物。已经鉴定了一种可变剪接的信使RNA，其编码在COOH末端截短的Max形式。这种DELTA-Max蛋白保留了与c-Myc复合物中的CACGTG基序结合的能力，但缺乏核定位信号和Max的推定调控结构域。当在大鼠胚胎成纤维细胞中进行myc-ras共转化试验时，Max抑制转化，而DELTA-Max增强转化。因此，max基因可以编码c-Myc功能的负调节因子和正调节因子。
Max is a basic-helix-loop-helix-leucine zipper protein capable of forming sequence-specific DNA binding complexes with Myc proteins. An alternatively spliced messenger RNA has been identified that encodes a form of Max truncated at the COOH-terminus. This DELTA-Max protein retained the ability to bind to the CACGTG motif in a complex with c-Myc but lacks the nuclear localization signal and the putative regulatory domain of Max. When tested in a myc-ras cotransformation assay in rat embryo fibroblasts, Max suppressed, whereas DELTA-Max enhanced, transformation. Thus, the maxgene may encode both a negative and a positive regulator of c-Myc function.