Biophysical Characterization of Aptamer-Target Interactions
Biophysical Characterization of Aptamer-Target Interactions
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DOI：
10.1007/10_2019_103
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发表时间：
2020-01-01
期刊：
影响因子：
--
通讯作者：
Schubert, Thomas
中科院分区：
文献类型：
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作者：
Plach, Maximilian;Schubert, Thomas
适体是形成明确的 3D 结构的单链核酸分子。适体通常以高亲和力和特异性与其配体结合。它们能够与各种配体相互作用：离子、小分子、肽、蛋白质、病毒、细菌，甚至细胞。因此，适体广泛用作诊断和制药中的传感器分子或靶向剂。作为在这些经济高价值领域使用的先决条件，必须对适配体的不同生物物理特性进行详细研究。最重要的是适体-靶标相互作用的基本结合参数，例如结合亲和力和动力学。目前，该领域使用了许多具有不同特征、特性和能力的生物物理方法。本章概述了当前研究适体和靶标之间相互作用的最先进技术，并讨论了它们的优点和缺点。此外，还将介绍影响任何适体表征策略的基本方面。最后，将讨论不同适体表征技术之间的结合数据的可比性问题。[图表]
Aptamers are single-stranded nucleic acid molecules forming welldefined 3D structures. Aptamers typically bind to their ligands with high affinity and specificity. They are capable of interacting with various kinds of ligands: ions, small molecules, peptides, proteins, viruses, bacteria, and even cells. Therefore, aptamers are in widespread use as sensor molecules or as targeting agents in diagnostics and pharmaceutics. As a prerequisite for their use in these economic high-value areas, aptamers must be studied in detail with respect to different biophysical characteristics. Of central importance are basic binding parameters of the aptamer-target interaction, such as binding affinity and kinetics. Numerous biophysical methods with different features, characteristics, and capabilities are used in the field today for this purpose.This chapter provides an overview of the current state-of-the-art technologies for studying interactions between aptamers and targets and discusses their advantages as well as drawbacks. Furthermore, essential aspects influencing any aptamer characterization strategy will be presented. Finally, issues of comparability of binding data between different aptamer characterization technologies will be discussed.[GRAPHICS]