THE SACCHAROMYCES-CEREVISIAE GENES (CMP1 AND CMP2) ENCODING CALMODULIN-BINDING PROTEINS HOMOLOGOUS TO THE CATALYTIC SUBUNIT OF MAMMALIAN PROTEIN PHOSPHATASE-2B
THE SACCHAROMYCES-CEREVISIAE GENES (CMP1 AND CMP2) ENCODING CALMODULIN-BINDING PROTEINS HOMOLOGOUS TO THE CATALYTIC SUBUNIT OF MAMMALIAN PROTEIN PHOSPHATASE-2B
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DOI：
10.1007/bf00260706
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发表时间：
1991-05-01
期刊：
影响因子：
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通讯作者：
MIYAKAWA, T
中科院分区：
文献类型：
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作者：
LIU, Y;ISHII, S;MIYAKAWA, T
以I-125标记的钙调素为探针，筛选酿酒酵母gt 11表达文库，获得了编码钙调素结合蛋白的酿酒酵母基因组克隆。 在克隆的酵母基因中，我们发现了两个密切相关的基因（CMP 1和CMP 2），它们编码与磷蛋白磷酸酶催化亚基同源的蛋白质。 推测的CMP 1蛋白（62999 Da）和CMP 2蛋白（68496 Da）含有23个氨基酸序列，与许多钙调蛋白调节蛋白中被鉴定为钙调蛋白结合位点的序列非常相似。 酵母基因编码与哺乳动物磷蛋白磷酸酶2B型（钙调神经磷酸酶）的催化亚基特别同源的蛋白质。 CMP 1和CMP 2基因的产物通过细胞提取物的免疫印迹分析分别鉴定为62000和64000 Da的蛋白质。 基因破坏实验表明，消除这些基因中的一个或两个对细胞活力没有影响，表明这些基因对正常细胞生长不是必需的。
Saccharomyces cerevisiae genomic clones that encode calmodulin-binding proteins were isolated by screening a lambda-gt11 expression library using I-125-labeled calmodulin as probe. Among the cloned yeast genes, we found two closely related genes (CMP1 and CMP2) that encode proteins homologous to the catalytic subunit of phosphoprotein phosphatase. The presumed CMP1 protein (62999 Da) and CMP2 protein (68496 Da) contain a 23 amino acid sequence very similar to those identified as calmodulin-binding sites in many calmodulin-regulated proteins. The yeast genes encode proteins especially homologous to the catalytic subunit of mammalian phosphorprotein phosphatase type 2B (calcineurin). The products of the CMP1 and CMP2 genes were identified by immunoblot analysis of cell extracts as proteins of 62000 and 64000 Da, respectively. Gene disruption experiments demonstrated that elimination of either or both of these genes had no effect on cell viability, indicating that these genes are not essential for normal cell growth.