Construction of black (Rubus occidentalis) and red (R. idaeus) raspberry linkage maps and their comparison to the genomes of strawberry, apple, and peach
Construction of black (Rubus occidentalis) and red (R. idaeus) raspberry linkage maps and their comparison to the genomes of strawberry, apple, and peach
复制标题
DOI：
10.1007/s00122-012-1835-5
复制
发表时间：
2012-07-01
影响因子：
5.4
通讯作者：
Gardiner, S. E.
中科院分区：
文献类型：
--
作者：
Bushakra, J. M.;Stephens, M. J.;Gardiner, S. E.
悬钩子属（Rubus）是蔷薇科（Rosaceae）悬钩子属（Rubus）植物的一种，约有600-800种，分布于世界各地。到目前为止，该属的遗传图谱主要由不可转移的标记，如扩增片段长度多态性。以欧洲悬钩子（Rubus occurentalis，96395 S1）为亲本，以欧洲悬钩子（Rubus occurentalis，R.利用Idaeus '莱瑟姆'构建了一个新的基于DNA序列标记的遗传图谱。本文所提供的遗传连锁图谱是由至少一个亲本图谱上的131个标记构建的。其中包括14个蔷薇科保守的直立组标记和60个新的树莓基因标记。34个已发表的树莓简单序列重复标记被用来比对新的地图，以公布的树莓地图。96395 S1遗传图谱由6个连锁群组成，覆盖309 cM，标记间平均10 cM;“莱瑟姆”遗传图谱由7个连锁群组成，覆盖561 cM，标记间平均5 cM。我们使用BLAST分析将用于设计悬钩子遗传作图引物对的正向引物序列与野草莓“夏威夷4”、苹果属“金冠”和李属“Lovell”的基因组序列进行比对。本文设计的同源性标记的比对结果表明悬钩子属和草莓属的基因组具有高度的同线性，且同线性随系统发育距离的增大而减小。我们的研究结果提供了前所未有的见解乌藨子的基因组进化从假定的祖先基因组的单一祖先共同的蔷薇科。
The genus Rubus belongs to the Rosaceae and is comprised of 600-800 species distributed world-wide. To date, genetic maps of the genus consist largely of non-transferable markers such as amplified fragment length polymorphisms. An F-1 population developed from a cross between an advanced breeding selection of Rubus occidentalis (96395S1) and R. idaeus 'Latham' was used to construct a new genetic map consisting of DNA sequence-based markers. The genetic linkage maps presented here are constructed of 131 markers on at least one of the two parental maps. The majority of the markers are orthologous, including 14 Rosaceae conserved orthologous set markers, and 60 new gene-based markers developed for raspberry. Thirty-four published raspberry simple sequence repeat markers were used to align the new maps to published raspberry maps. The 96395S1 genetic map consists of six linkage groups (LG) and covers 309 cM with an average of 10 cM between markers; the 'Latham' genetic map consists of seven LG and covers 561 cM with an average of 5 cM between markers. We used BLAST analysis to align the orthologous sequences used to design primer pairs for Rubus genetic mapping with the genome sequences of Fragaria vesca 'Hawaii 4', Malus x domestica 'Golden Delicious', and Prunus 'Lovell'. The alignment of the orthologous markers designed here suggests that the genomes of Rubus and Fragaria have a high degree of synteny and that synteny decreases with phylogenetic distance. Our results give unprecedented insights into the genome evolution of raspberry from the putative ancestral genome of the single ancestor common to Rosaceae.