An Integrated Approach to Explore Composition and Dynamics of Cholesterol- rich Membrane Microdomains in Sexual Stages of Malaria Parasite
An Integrated Approach to Explore Composition and Dynamics of Cholesterol- rich Membrane Microdomains in Sexual Stages of Malaria Parasite
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DOI：
10.1074/mcp.m117.067041
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发表时间：
2017-10-01
影响因子：
7
通讯作者：
Ponzi, Marta
中科院分区：
文献类型：
--
作者：
Fratini, Federica;Raggi, Carla;Ponzi, Marta
包括脂筏在内的膜微区参与重要的生理和病理过程，并参与细胞内病原体的进入。这些组件，富含胆固醇和鞘脂，形成高度动态的，液体有序的相，可以从本体膜分离，由于其耐溶解的非离子型洗涤剂。为了表征啮齿类疟原虫伯氏疟原虫性阶段耐洗涤剂膜的复杂性和动态性，在这里，我们提出了一个基于蛋白质组学，脂质分析和生物信息学的筏组件的综合研究。该分析揭示了与这些特化组装体相关的意外异质性和未探索的途径。蛋白质-蛋白质关系和蛋白质-脂质共现通过多组分网络描述。所提出的方法可以广泛应用于生理和/或病理条件下不同背景和扰动下的几乎所有细胞类型。
Membrane microdomains that include lipid rafts, are involved in key physiological and pathological processes and participate in the entry of endocellular pathogens. These assemblies, enriched in cholesterol and sphingolipids, form highly dynamic, liquid-ordered phases that can be separated from the bulk membranes thanks to their resistance to solubilization by nonionic detergents. To characterize complexity and dynamics of detergent-resistant membranes of sexual stages of the rodent malaria parasite Plasmodium berghei, here we propose an integrated study of raft components based on proteomics, lipid analysis and bioinformatics. This analysis revealed unexpected heterogeneity and unexplored pathways associated with these specialized assemblies. Protein-protein relationships and protein-lipid co-occurrence were described through multi-component networks. The proposed approach can be widely applied to virtually every cell type in different contexts and perturbations, under physiological and/or pathological conditions.