Fibronectin-mediated cellular adhesion to vascular subendothelial matrices.
Fibronectin-mediated cellular adhesion to vascular subendothelial matrices.
复制标题
纤连蛋白介导的细胞粘附至血管内皮下基质。
DOI：
10.1016/0014-4827(81)90473-0
复制
发表时间：
1981
影响因子：
3.7
通讯作者：
Hoffstein,ST
中科院分区：
文献类型：
--
作者：
Pearlstein,E;Hoffstein,ST
虽然纤连蛋白已被很好地确立为体外细胞粘附的介体，并因此被假定在体内发挥作用，但纤连蛋白在体内介导细胞粘附的能力尚未得到证实。因此，我们已经开发了一种方法，用于定量的细胞附着到血管内皮和内皮下基质，并已使用它来评估纤连蛋白在促进纤连蛋白缺陷的转化细胞附着到这个网站的作用。虽然只有10%的细胞粘附到完整的内皮衬里，这种附着增加8-10倍时，衬里细胞被删除的程序，允许保留内皮下纤连蛋白。当衬里细胞被去除更激烈的程序，也去除了内皮下纤连蛋白，转化细胞的粘附下降到6%。然而，转化细胞对基底膜的粘附并不完全依赖于纤连蛋白。治疗与抗纤连蛋白抗血清，完全抑制这些细胞的粘附在体外生长的内皮细胞或成纤维细胞的细胞外基质可以抑制附着到基膜只有60%。这些数据表明，细胞粘附到由培养中的细胞产生的微渗出物可能不是研究体内细胞粘附的适当模型。我们在这里描述的方法可以提供一个更现实的模型，并可用于研究各种细胞基质相互作用。
Although fibronectin has been well established as a mediator of cell adherence in vitro and has therefore been presumed to play a role as such in vivo, the ability of fibronectin, laid down in vivo, to mediate cell attachment, has not been demonstrated. We have therefore developed a method for quantitating the attachment of cells to vascular endothelium and subendothelial matrix and have used it to assess the role of fibronectin in promoting the attachment of a fibronectin-deficient transformed cell to this site. Whereas only 10% of cells adhered to intact endothelial linings, this attachment increased 8–10-fold when the lining cells were removed by procedures which permitted the retention of subendothelial fibronectin. When lining cells were removed by more drastic procedures which also removed subendothelial fibronectin, adherence of transformed cells was decreased to 6%. Adherence of transformed cells to the basal lamina was not, however, exclusively dependent upon fibronectin. Treatment with antifibronectin antiserum which completely inhibited adherence of these cells to the extracellular matrices of endothelial cells or fibroblasts grown in vitro could inhibit attachment to basal lamina by only 60%. The data suggest that cellular adhesion to microexudates produced by cells in culture may not be an adequate model with which to study cell adhesion in vivo. The method we describe here may provide a more realistic model and could be used to study a variety of cell-substrate interactions.