Esculentin 1-21: a linear antimicrobial peptide from frog skin with inhibitory effect on bovine mastitis-causing bacteria
Esculentin 1-21: a linear antimicrobial peptide from frog skin with inhibitory effect on bovine mastitis-causing bacteria
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DOI：
10.1002/psc.1148
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发表时间：
2009-09-01
影响因子：
2.1
通讯作者：
Mangoni, Maria Luisa
中科院分区：
文献类型：
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作者：
Enrique Islas-Rodriguez, Alfonso;Marcellini, Ludovica;Mangoni, Maria Luisa
乳腺炎或乳腺炎症是全世界奶牛最常见和最昂贵的疾病。虽然压力和身体伤害可能会引起乳房炎症，但细菌或其他微生物感染仍然是主要原因，抗生素输注是主要治疗方法。然而，多重耐药病原体的出现和生产的牛奶被抗生素污染，已成为牲畜的严重威胁。因此，迫切需要发现具有新作用模式的新治疗剂。基因编码的抗菌肽是所有生物体的第一道防线，被认为是开发新的抗感染药物的有希望的候选者。本文报道了蛙皮AMP esculentin 1-21 [ESC（1-21）]的体外和动物模型抗菌活性，沿着了可能的作用机制。我们的数据显示，这种肽（i）对最常见的乳腺炎致病微生物（例如无乳链球菌）具有高度效力;和（ii）在体内具有活性，在肽治疗1周后，导致奶牛乳腺炎临床阶段明显消退。生物物理特性表明，该肽在微生物模拟膜中采用α-螺旋结构，并且能够渗透S.以剂量依赖性方式无乳。总体而言，这些数据表明，ESC（1-21）是一种有吸引力的AMP，可用于未来设计治疗牛乳腺炎的新抗生素。版权所有（C）2009欧洲肽协会和John Wiley & Sons，Ltd.
Mastitis, or inflammation of the mammary gland, is the most common and expensive illness of dairy cows throughout the world. Although stress and physical injuries may give rise to inflammation of the udders, infections by bacteria or other microorganisms remain the major cause, and infusion of antibiotics is the main treatment approach. However, the increased emergence of multidrug-resistant pathogens and the production of milk contaminated with antibiotics has become a serious threat in the livestock. Hence, there is an urgent need for the discovery of new therapeutic agents with a new mode of action. Gene-encoded AMPs, which represent the first line of defence in all living organisms, are considered as promising candidates for the development of new anti-infective agents. This paper reports on the antibacterial activities in vitro and in an animal model, of the frog skin AMP esculentin 1-21 [Esc(1-21)], along with a plausible mode of action. Our data revealed that this peptide (i) is highly potent against the most common mastitis-causing microbes (e.g. Streptococcus agalactiae); and (ii) is active in vivo, causing a visible regression of the clinical stage of mastitis in dairy cows, after I week of peptide treatment. Biophysical characterisation revealed that the peptide adopts an alpha-helical structure in microbial mimicking membranes and is able to permeate the membrane of S. agalactiae in a dose-dependent manner. Overall, these data suggest Esc(1-21) as an attractive AMP for the future design of new antibiotics to cure mastitis in cattle. Copyright (C) 2009 European Peptide Society and John Wiley & Sons, Ltd.