2 TYPES OF NORMAL HUMAN BREAST EPITHELIAL-CELLS DERIVED FROM REDUCTION MAMMAPLASTY - PHENOTYPIC CHARACTERIZATION AND RESPONSE TO SV40 TRANSFECTION
2 TYPES OF NORMAL HUMAN BREAST EPITHELIAL-CELLS DERIVED FROM REDUCTION MAMMAPLASTY - PHENOTYPIC CHARACTERIZATION AND RESPONSE TO SV40 TRANSFECTION
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DOI：
10.1093/carcin/16.3.531
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发表时间：
1995-03-01
期刊：
影响因子：
4.7
通讯作者：
CHANG, CC
中科院分区：
文献类型：
--
作者：
KAO, CY;NOMATA, K;CHANG, CC
一种培养方法，生长两种形态上可区分的正常人乳房上皮细胞类型来自减少乳房成形术已被开发。I型细胞的特征在于更多变的细胞形状，光滑的细胞集落边界，上皮膜抗原（EMA）和角蛋白18的表达和角蛋白14和α 6整合素的非表达。另外，与之相反，I型细胞被胎牛血清（FBS）刺激生长并且缺乏间隙连接细胞间通讯（GJIC）。Ⅱ型细胞形态均一，表达角蛋白14和α 6整合素，不表达EMA和角蛋白18。I型细胞可以被霍乱毒素诱导以将它们的形态改变为II型细胞形态。因此，I型细胞在抗原性上类似于腔上皮细胞，而II型细胞更类似于基底上皮细胞。用SV 40 DNA转染I型和II型细胞，通过SV 40转染从I型和II型细胞中获得寿命延长的克隆。一些（2/9）SV 40转染的I型细胞克隆变得永生（>100累积群体倍增水平），而没有（0/8）SV 40转染的II型细胞克隆变得永生。SV 40转染的I型和II型细胞衍生的延长寿命克隆和永生细胞系在EMA、角蛋白14和角蛋白18表达以及GJIC方面在表型上类似于其亲本细胞。每个（9/9）SV 40转染的I型细胞克隆在软琼脂中生长;没有（0/8）SV 40转染的II型细胞克隆能够在软琼脂中生长。这些结果提供的证据表明，正常的人乳腺上皮细胞，来自乳房缩小术，可以分为两种形态和抗原不同的细胞类型，这两种不同的细胞类型显着不同的反应致癌（SV 40）刺激。
A culture method to grow two morphologically distinguishable normal human breast epithelial cell types derived from reduction mammoplasty has been developed. Type I cells were characterized by a more variable cell shape, smooth cell colony boundaries, the expression of epithelial membrane antigen (EMA) and keratin 18 and the nonexpression of keratin 14 and alpha 6 integrin. In addition, the Type I cells were growth stimulated by fetal bovine serum (FBS) and were deficient in gap junctional intercellular communication (GJIC), In contrast. Type II cells were characterized by a uniform cell shape, expression of keratin 14 and alpha 6 integrin and the non-expression of EMA and keratin 18, In addition, Type II cells were growth inhibited by FBS and were proficient in GJIC. Type I cells can be induced by cholera toxin to change their morphology to a Type II cell morphology. Hence, Type I cells antigenically resemble luminal epithelial cells, while the Type II cells more closely resemble basal epithelial cells. Type I and Type II cells were transfected with SV40 DNA, Clones with extended lifespans were obtained from both Type I and Type II cells by SV40 transfection. Some (2/9) of the SV40-transfected Type I cell clones became immortal (>100 cumulative population doubling level), whereas none (0/8) of the SV40-transfected Type II cell clones became immortal. The SV40-transfected Type I and Type II cell-derived extended life clones and immortal cell lines phenotypically resembled their parental cells with respect to EMA, keratin 14 and keratin 18 expression and GJIC. Each (9/9) of the SV40 transfected Type I cell clones grew in soft agar; none (0/8) of the SV40-transfected Type II cell clones were capable of growing in soft agar. These results provide evidence that normal human breast epithelial cells, derived from reduction mammoplasty, can be separated into two morphologically and antigenically different cell types and that these two different cell types significantly differ in their response to an oncogenic (SV40) stimulus.