A polydopamine coated nanoscale FeS theranostic platform for the elimination of drug-resistant bacteria via photothermal-enhanced Fenton reaction
A polydopamine coated nanoscale FeS theranostic platform for the elimination of drug-resistant bacteria via photothermal-enhanced Fenton reaction
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DOI：
10.1016/j.actbio.2022.07.046
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发表时间：
2022-08-31
影响因子：
9.7
通讯作者：
Liu, Fengxiang
中科院分区：
文献类型：
--
作者：
Li, Fupeng;Huang, Kai;Liu, Fengxiang
由耐药细菌引起的感染对人类健康构成极大威胁。非抗生素依赖的抗菌策略已成为研究的重点。其中，基于化学动态治疗(CDT)的治疗系统通过酶催化产生羟基自由基，在抗菌方面取得了巨大的成功。然而，有限的Fenton反应动力学、较差的渗透性和较短的羟基半衰期影响了CDT的抗菌效果。此外，感染的早期诊断困难导致滥用药物和延误治疗。在本论文中，我们合成了聚多巴胺包被的硫化亚铁吸附了光热处理(PTT)增强的CDT的次氯酸盐响应探针的热敏平台。该探头组件用于感染的早期诊断。PTT不仅通过高温灭活细菌，而且还加速了Fenton反应，产生了更多的中心点OH。体外抗菌实验表明，该多功能抗菌平台具有广泛的抗菌谱，包括耐甲氧西林金黄色葡萄球菌(MRSA)、耐药大肠埃希菌(DR E.coli)和铜绿假单胞菌(P。此外，体内抗菌实验表明，纳米颗粒可以有效地修复金黄色葡萄球菌感染的全层皮肤缺损，细胞毒性可以忽略不计。本研究提出了一种高效、多功能的细菌感染治疗平台，为抗生素耐药性的治疗提供了有效的协同抗菌策略。
Infections caused by drug-resistant bacteria pose a great threat to human health. Non-antibioticdependent antibacterial strategies have become the focus of research. Among them, chemical dynamic treatment-based (CDT) therapeutic systems, which catalyze the production of hydroxyl radicals by enzymes, have achieved tremendous success for antibacterial purposes. However, limited kinetics of the Fenton reaction, poor permeability, and short half-life of hydroxyl radicals compromise the antibacterial effects of CDT. In addition, difficulties in the early diagnosis of infection lead to drug abuse and delayed treatment. Herein, a polydopamine coated ferrous sulfide theranostic platform adsorbing a hypochlorite responsive probe with photothermal treatment (PTT) enhanced CDT was synthesized. The probe component was used for the early diagnosis of infection. PTT not only inactivated bacteria by hyperthermia but also accelerated the Fenton reaction to produce more center dot OH. In vitro antibacterial experiments demonstrated that the multifunctional theranostic platform has a broad antibacterial spectrum, including methicillin-resistant Staphylococcus aureus (MRSA), drug-resistant Escherichia coli (DR E. coli ), and Pseudomonas aeruginosa ( P. aeruginosa ). In addition, in vivo antibacterial experiments demonstrated that nanoparticles could effectively rescue S. aureus- infected full-thickness skin defects with negligible cytotoxicity. This study proposes an efficient and multifunctional theranostic platform for bacterial infection, providing an effective synergistic antibacterial strategy for the treatment of antibiotic resistance.