ACCURACY OF MAPPING QUANTITATIVE TRAIT LOCI IN AUTOGAMOUS SPECIES
ACCURACY OF MAPPING QUANTITATIVE TRAIT LOCI IN AUTOGAMOUS SPECIES
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DOI：
10.1007/bf00227388
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发表时间：
1992-09-01
影响因子：
5.4
通讯作者：
VANOOIJEN, JW
中科院分区：
文献类型：
--
作者：
VANOOIJEN, JW
大量分子标记的连锁图谱的发展促进了对数量性状相关基因定位方法的探索。Lander和Botstein（1989）提出了一种很有前途的方法，利用相邻标记对获得关于封闭染色体片段内QTL存在的最大连锁信息。本文通过计算机模拟研究了这种方法的精度。结果表明，有一个合理的概率检测QTL解释至少5%的总方差。为此目的，需要至少200个回交或F2个体的群体。个体数和QTL基因型效应的相对大小是决定定位精度的重要因素。平均而言，具有5%或10%解释方差的QTL分别定位在40或20厘摩的间隔上。当然，具有较大基因型效应的QTL将被更精确地定位。然而，必须指出的是，间隔长度是相当可变的。
The development of linkage maps with large numbers of molecular markers has stimulated the search for methods to map genes involved in quantitative traits (QTLs). A promising method, proposed by Lander and Botstein (1989), employs pairs of neighbouring markers to obtain maximum linkage information about the presence of a QTL within the enclosed chromosomal segment. In this paper the accuracy of this method was investigated by computer simulation. The results show that there is a reasonable probability of detecting QTLs that explain at least 5% of the total variance. For this purpose a minimum population of 200 backcross or F2 individuals is necessary. Both the number of individuals and the relative size of the genotypic effect of the QTL are important factors determining the mapping precision. On the average, a QTL with 5% or 10% explained variance is mapped on an interval of 40 or 20 centiMorgans, respectively. Of course, QTLs with a larger genotypic effect will be located more precisely. It must be noted, however, that the interval length is rather variable.