Arf6 regulates EGF-induced internalization of E-cadherin in breast cancer cells.
Arf6 regulates EGF-induced internalization of E-cadherin in breast cancer cells.
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Arf6 调节 EGF 诱导的乳腺癌细胞中 E-钙粘蛋白的内化
DOI：
10.1186/s12935-015-0159-3
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发表时间：
2015
影响因子：
5.8
通讯作者：
Du J
中科院分区：
文献类型：
--
作者：
Xu R;Zhang Y;Gu L;Zheng J;Cui J;Dong J;Du J
E-钙粘蛋白内化促进粘附连接的溶解，并促进肿瘤细胞上皮间质转化（EMT）和迁移。我们以前的研究结果表明，Arf 6在EGF刺激后对乳腺癌细胞发挥促迁移作用。尽管EGF信号刺激乳腺癌细胞的EMT，但Arf 6对EGF处理下乳腺癌细胞的E-钙粘蛋白内化的影响仍有待确定。在这里，我们发现EGF剂量依赖性地刺激MCF-7细胞的E-cadherin内化，在50 ng/ml时效果最大。同时，EGF处理显著增加Arf 6活化。Arf 6参与了E-cadherin的复合物，当Arf 6的活性增加时，更多的E-cadherin被Arf 6拉下。免疫印迹和免疫荧光检测显示，转染Arf 6-T27 N或Arf 6 siRNA的乳腺癌细胞抑制EGF诱导的E-cadherin内化。综上所述，我们的研究表明，Arf 6激活在EGF诱导的E-cadherin内化中起着潜在的作用，为Arf 6促进乳腺癌细胞转移提供了新的机制。本文的在线版本（doi：10.1186/s12935-015-0159-3）包含补充材料，可供授权用户使用。
E-cadherin internalization facilitates dissolution of adherens junctions and promotes tumor cell epithelial-mesenchymal transition (EMT) and migration. Our previous results have shown that Arf6 exerts pro-migratory action in breast cancer cells after EGF stimulation. Despite the fact that EGF signaling stimulates EMT of breast cancer cells, the effect of Arf6 on internalization of E-cadherin of breast cancer cells under EGF treatment remains to be determined. Here, we showed that EGF dose-dependently stimulated E-cadherin internalization by MCF-7 cells with the maximal effect at 50 ng/ml. Meanwhile, EGF treatment markedly increased Arf6 activation. Arf6 was involved in complexes of E-cadherin, and more E-cadherin was pulled down with Arf6 when the activity of the latter was increased. Immunoblotting and immunofluorescence assays showed that transfection breast cancer cells with Arf6-T27N or Arf6 siRNA suppressed EGF-induced E-cadherin internalization. Taken together, our study demonstrated that Arf6 activation plays a potential role in EGF-induced E-cadherin internalization, providing new mechanism underlying the effect of Arf6 on promoting breast cancer cell metastasis. The online version of this article (doi:10.1186/s12935-015-0159-3) contains supplementary material, which is available to authorized users.
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影响因子：
3.3
作者：
Bach AS;Enjalbert S;Comunale F;Bodin S;Vitale N;Charrasse S;Gauthier-Rouvière C
通讯作者：
Gauthier-Rouvière C
影响因子：
11.2
作者：
Onder, Tamer T.;Gupta, Piyush B.;Weinberg, Robert A.
通讯作者：
Weinberg, Robert A.
影响因子：
3.8
作者：
Knudsen, Erik S.;Ertel, Adam;Witkiewicz, Agnieszka K.
通讯作者：
Witkiewicz, Agnieszka K.
影响因子：
2.5
作者：
Hardy, Katharine M.;Booth, Brian W.;Hendrix, Mary J. C.;Salomon, David S.;Strizzi, Luigi
通讯作者：
Strizzi, Luigi
GEP100/Arf6 是人肝癌 HepG2 细胞中表皮生长因子诱导的 ERK/Rac1 信号传导和细胞迁移所必需的
影响因子：
3.7
作者：
Hu Z;Du J;Yang L;Zhu Y;Yang Y;Zheng D;Someya A;Gu L;Lu X
通讯作者：
Lu X