Synthesis and characterization of polyamidoamine dendrimer-coated multi-walled carbon nanotubes and their application in gene delivery systems
Synthesis and characterization of polyamidoamine dendrimer-coated multi-walled carbon nanotubes and their application in gene delivery systems
复制标题
聚酰胺胺树枝状大分子包覆的多壁碳纳米管的合成、表征及其在基因传递系统中的应用
DOI：
10.1088/0957-4484/20/12/125101
复制
发表时间：
2009-03-25
期刊：
影响因子：
3.5
通讯作者：
Hu, Guohan
中科院分区：
文献类型：
--
作者：
Pan, Bifeng;Cui, Daxiang;Hu, Guohan
为了提高碳纳米管携带基因进入人体癌细胞的数量和传递效率，制备了不同代聚酰胺-胺树枝状大分子修饰的多壁碳纳米管（dMNTs），并通过高分辨透射电子显微镜、原子力显微镜、x射线光电子能谱、拉曼光谱、傅立叶变换红外光谱和热重分析对其进行了表征，揭示了在碳纳米管的表面上存在树枝状聚合物。将dMNTs与FITC标记的反义c-myc寡核苷酸（asODN）完全偶联后，与人乳腺癌细胞系MCF-7、MDA-MB-435和肝癌细胞系HepG 2共同孵育，激光共聚焦显微镜观察证实，asODN-dMNTs复合物在15 min内进入肿瘤细胞。这些复合物呈时间和剂量依赖性地抑制细胞生长，并下调c-myc基因和C-Myc蛋白的表达。与CNT-NH 2-asODN和dendrimer-asODN复合物相比，第5代dendrimer-mNT-asODN复合物的转染效率和对肿瘤细胞的抑制作用最高。通过dMNTs的细胞内基因转运和摄取对于哺乳动物细胞系应该是通用的。dMNTs在肿瘤治疗的基因或药物递送以及分子成像等方面具有潜在的应用前景。
With the aim of improving the amount and delivery efficiency of genes taken by carbon nanotubes into human cancer cells, different generations of polyamidoamine dendrimer modified multi-walled carbon nanotubes (dMNTs) were fabricated, and characterized by high-resolution transmission electron microscopy, atomic force microscopy, x-ray photoelectron spectroscopy, Raman spectroscopy, Fourier transform infrared spectroscopy and thermogravimetric analysis, revealing the presence of dendrimer capped on the surface of carbon nanotubes. The dMNTs fully conjugated with FITC-labeled antisense c-myc oligonucleotides (asODN), those resultant asODN–dMNTs composites were incubated with human breast cancer cell line MCF-7 cells and MDA-MB-435 cells, and liver cancer cell line HepG2 cells, and confirmed to enter into tumor cells within 15 min by laser confocal microscopy. These composites inhibited the cell growth in time- and dose-dependent means, and down-regulated the expression of the c-myc gene and C-Myc protein. Compared with the composites of CNT–NH2–asODN and dendrimer–asODN, no. 5 generation of dendrimer-modified MNT–asODN composites exhibit maximal transfection efficiencies and inhibition effects on tumor cells. The intracellular gene transport and uptake via dMNTs should be generic for the mammalian cell lines. The dMNTs have potentials in applications such as gene or drug delivery for cancer therapy and molecular imaging.