Heterotrophy of filamentous oleaginous microalgae Tribonema minus for potential production of lipid and palmitoleic acid.
Heterotrophy of filamentous oleaginous microalgae Tribonema minus for potential production of lipid and palmitoleic acid.
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DOI：
10.1016/j.biortech.2017.05.045
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发表时间：
2017-09
影响因子：
11.4
通讯作者：
Wenjun Zhou;H. Wang;Lin Chen;Wentao Cheng;Tianzhong Liu
中科院分区：
文献类型：
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作者：
Wenjun Zhou;H. Wang;Lin Chen;Wentao Cheng;Tianzhong Liu
异养发酵和高附加值副产物的生产被认为是提高微藻生物燃料经济性和商业化生产可行性的有效途径。本文报道了异养培养小拟谷盗生产油脂和棕榈油酸的研究。首先，研究了T.首次确定了负碳源对生物量和油脂产量有显著的促进作用，并筛选出葡萄糖和尿素作为最佳碳源和氮源。并对培养条件和营养盐浓度进行了优化。当一次添加80 g L-1葡萄糖时，获得最高的生物量和油脂生产率，分别为30.8 g L-1和730 mg L-1d-1。此外，确定2 g L− 1尿素、0.8 g L− 1 K2 HPO 4、24 mg L− 1柠檬酸铁铵、初始pH 6和温度27 °C为异养生长和脂质生产的适当条件。
Heterotrophic fermentation and high valuable co-product producing are thought to be effective ways to improve the economic viability and feasibility of commercial production of microalgae biofuels. This work reported the heterotrophic cultivation ofTribonema minusfor lipid and palmitoleic acid (a novel functional fatty acid) production. Firstly, the heterotrophic ability ofT. minuswas identified for the first time with significant promotion in biomass and lipid productivity, and glucose and urea were then selected as the optimal carbon and nitrogen sources. Moreover, nutrient concentrations and culture conditions were optimized. Highest biomass and lipid productivity of 30.8 g L−1and 730 mg L−1d−1were obtained respectively by adding 80 g L−1glucose at once. In addition, 2 g L−1urea, 0.8 g L−1K2HPO4, 24 mg L−1ammonium ferric citrate, initial pH of 6, and temperature of 27 °C were determined as the appropriate conditions for heterotrophic growth and lipid production.