The YPWM motif links Antennapedia to the basal transcriptional machinery
The YPWM motif links Antennapedia to the basal transcriptional machinery
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DOI：
10.1242/dev.018028
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发表时间：
2008-05-01
期刊：
影响因子：
4.6
通讯作者：
Gehring, Walter J.
中科院分区：
文献类型：
--
作者：
Prince, Frederic;Katsuyama, Tomonori;Gehring, Walter J.
HOX基因指定沿着胚胎前后轴的片段身份沿着。它们编码的转录因子具有高度保守的同源结构域和YPWM基序，位于氨基末端。尽管它们在体内的功能高度多样化，HOX蛋白在体外显示出相似的生化特性，这引起了如何实现特异性的问题。在我们的研究中，我们调查的重要性，在成年果蝇的同源异型转化的Antp（YPWM基序）。通过异位过表达，果蝇的头部结构可以转化为第二胸节的结构，如触角转化为第二腿，头囊转化为胸部（背），眼睛转化为翅膀。我们发现，YPWM基序是绝对需要的眼睛到翅膀的转换。使用酵母双杂交系统，我们能够确定一种新的ANTP相互作用蛋白，Bric-a-brac相互作用蛋白2（BIP 2），它特异性地与ANTP的YPWM基序在体外相互作用，以及在体内，将眼睛转化为翅膀组织。BIP 2是一种TATA结合蛋白相关因子（也称为dTAFII 3），将ANTP与基础转录机制联系起来。
HOX genes specify segment identity along the anteroposterior axis of the embryo. They code for transcription factors harbouring the highly conserved homeodomain and a YPWM motif, situated amino terminally to it. Despite their highly diverse functions in vivo, HOX proteins display similar biochemical properties in vitro, raising the question of how this specificity is achieved. In our study, we investigated the importance of the Antennapedia (Antp) YPWM motif for homeotic transformations in adult Drosophila. By ectopic overexpression, the head structures of the fly can be transformed into structures of the second thoracic segment, such as antenna into second leg, head capsule into thorax (notum) and eye into wing. We found that the YPWM motif is absolutely required for the eye-to-wing transformation. Using the yeast two-hybrid system, we were able to identify a novel ANTP-interacting protein, Bric-a-brac interacting protein 2 (BIP2), that specifically interacts with the YPWM motif of ANTP in vitro, as well as in vivo, transforming eye to wing tissue. BIP2 is a TATA-binding protein associated factor (also known as dTAFII3) that links ANTP to the basal transcriptional machinery.