SPECIFIC EXPRESSION OF THE HUMAN CELLULAR FPS FES-ENCODED PROTEIN NCP92 IN NORMAL AND LEUKEMIC MYELOID CELLS
SPECIFIC EXPRESSION OF THE HUMAN CELLULAR FPS FES-ENCODED PROTEIN NCP92 IN NORMAL AND LEUKEMIC MYELOID CELLS
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DOI：
10.1073/pnas.82.8.2379
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
HANAFUSA, H
中科院分区：
文献类型：
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作者：
FELDMAN, RA;GABRILOVE, JL;HANAFUSA, H
针对富士那美肉瘤病毒转化蛋白P140保守激酶结构域氨基酸序列的合成肽的抗体和一种退化肿瘤抗血清均能识别禽[鸡胚成纤维细胞]和哺乳动物细胞的c-fps/fes基因产物。抗肽抗体还识别出一种94千道尔顿（kDa）蛋白，该蛋白在结构和组织分布上与c-fps/fes产物相关，但又不同。一种92kda蛋白NCP92与先前鉴定的禽类c-fps/fes蛋白NCP98结构相似，其相关酪氨酸激酶活性和组织分布相似，是哺乳动物的对应物。NCP92在组织巨噬细胞和骨髓中含量最高。在骨髓中，NCP92的表达仅限于单核/巨噬细胞和粒细胞谱系。对不同细胞系的小鼠和人造血肿瘤的分析证实了NCP92的表达仅限于这些细胞类型：NCP92在粒细胞和单核细胞来源的白血病细胞中呈阳性，而在b淋巴细胞、t淋巴细胞和红系肿瘤细胞中不呈阳性。NCP92的表达似乎与骨髓细胞的分化能力和对某些集落刺激因子的反应有关。
Both an antibody directed against a synthetic peptide representing an amino acid sequence of the conserved kinase domain of transforming protein P140 of Fujinami sarcoma virus and a regressing tumor antiserum recognized the products of the c-fps/fes genes of both avian [chicken embryo fibroblasts] and mammalian cells. The anti-peptide antibody also recognized a 94-kilodalton [kDa] protein that was related to but distinct from the c-fps/fes product in structure and in tissue distribution. A 92-kDa protein, NCP92, was the mammalian counterpart of the previously identified avian c-fps/fes protein NCP98 by its structural similarity to NCP98, its associated tyrosine kinase activity, and its similar tissue distribution. The highest levels of NCP92 were found in tissue macrophages and in bone marrow. In bone marrow NCP92 expression was restricted to cells of the monocyte/macrophage and granulocyte lineages. That the expression of NCP92 is limited to these cell types was confirmed by the analysis of murine and human hematopoietic tumors representing different cell lineages: NCP92 was positive in leukemic cells of granulocytic and monocytic origin but not in B-lymphocytic, T-lymphocytic or erythroid tumor cells. The expression of NCP92 seems to be related to the capacity of myeloid cells to differentiate and to respond to certain colony-stimulating factors.