A UBIQUITIN CONJUGATING ENZYME ENCODED BY AFRICAN SWINE FEVER VIRUS
A UBIQUITIN CONJUGATING ENZYME ENCODED BY AFRICAN SWINE FEVER VIRUS
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DOI：
10.1002/j.1460-2075.1992.tb05058.x
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发表时间：
1992-01-01
期刊：
影响因子：
11.4
通讯作者：
DIXON, LK
中科院分区：
文献类型：
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作者：
HINGAMP, PM;ARNOLD, JE;DIXON, LK
蛋白质的翻译后修饰通过泛素的共价连接发生在所有真核生物中，通过多步骤的过程。E2或泛素结合（UBC）酶家族催化该过程的一个步骤，并且这些酶已经涉及几种不同的调节功能。本文报道了非洲猪瘟病毒（ASFV）编码的一个基因的序列，该基因与UBC酶具有高度同源性。该ASFV编码的酶在大肠杆菌中表达时具有UBC活性，因为其在纯化的泛素活化酶（E1）和ATP存在下与[I-125]泛素形成硫酯键，随后将[I-125]泛素转移至特定的蛋白质底物。这些底物包括组蛋白、泛素和UBC酶本身。ASFV编码的UBC酶在结构和酶活性上与酵母泛素缀合酶UBC 2和UBC 3相似。这是第一次报告的病毒编码的功能活性UBC酶，并提供了一个例子，利用宿主的调节机制的病毒。
The post-translational modification of proteins by covalent attachment of ubiquitin occurs in all eukaryotes by a multi-step process. A family of E2 or ubiquitin conjugating (UBC) enzymes catalyse one step of this process and these have been implicated in several diverse regulatory functions. We report here the sequence of a gene encoded by African swine fever virus (ASFV) which has high homology with UBC enzymes. This ASFV encoded enzyme has UBC activity when expressed in Escherichia coli since it forms thiolester bonds with [I-125]ubiquitin in the presence of purified ubiquitin activating enzyme (E1) and ATP, and subsequently transfers [I-125]ubiquitin to specific protein substrates. These substrates include histones, ubiquitin and the UBC enzyme itself. The ASFV encoded UBC enzyme is similar in structure and enzyme activity to the yeast ubiquitin conjugating enzymes UBC2 and UBC3. This is the first report of a virus encoding a functionally active UBC enzyme and provides an example of the exploitation of host regulatory mechanisms by viruses.