‘Candidatus Phytoplasma noviguineense’, a novel taxon associated with Bogia coconut syndrome and banana wilt disease on the island of New Guinea
‘Candidatus Phytoplasma noviguineense’, a novel taxon associated with Bogia coconut syndrome and banana wilt disease on the island of New Guinea
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“Candidatus Phytoplasma noviguineense”，一种与新几内亚岛博吉亚椰子综合症和香蕉枯萎病相关的新分类单元
DOI：
10.1099/ijsem.0.002480
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发表时间：
2018
影响因子：
2.8
通讯作者：
Namba Shigetou
中科院分区：
文献类型：
--
作者：
Miyazaki Akio;Shigaki Toshiro;Koinuma Hiroaki;Iwabuchi Nozomu;Rauka Gou Bue;Kembu Alfred;Saul Josephine;Watanabe Kiyoto;Nijo Takamichi;Maejima Kensaku;Yamaji Yasuyuki;Namba Shigetou
博吉亚椰子综合征（BCS）是一种与植原体相关的致死性黄化（LY）型疾病，严重威胁着世界各地的椰子种植。它最近出现，并在新几内亚岛的北方地区迅速蔓延。比较了不同地区的BCS相关植原体的16 S rRNA基因序列，发现以BCS-BoR为代表的菌株具有较高的同源性。16 S rRNA基因序列分析表明，BCS-BoR与其它种的相似性小于97.5%，最高为96.08%（与LY株; GenBank登录号U18747）。 这一结果表明，根据IRPCM的建议，一个新的分类单元的BCS植原体的必要性和适当性。对16 S rRNA基因序列进行了系统发育分析，结果表明BCS-BoR和其他LY相关植原体组成了一个单系簇。新几内亚岛上与香蕉枯萎病和槟榔黄叶病相关的植原体与BCS-BoR具有很高的相似性，与BCS植原体密切相关。基于16 S rRNA基因序列的独特性，以BCS-BoR株（GenBank登录号LC 228755）为参考株，将新几内亚岛植原体命名为“Ca.Phytoplasma noviguineense”。详细描述了新的分类群，包括相关疾病的症状和thesecY基因和roperon的额外遗传特征的信息。
Bogia coconut syndrome (BCS) is one of the lethal yellowing (LY)-type diseases associated with phytoplasma presence that are seriously threatening coconut cultivation worldwide. It has recently emerged, and is rapidly spreading in northern parts of the island of New Guinea. BCS-associated phytoplasmas collected in different regions were compared in terms of 16S rRNA gene sequences, revealing high identity among them represented by strain BCS-BoR. Comparative analysis of the 16S rRNA gene sequences revealed that BCS-BoRshared less than a 97.5 % similarity with other species of ‘CandidatusPhytoplasma’, with a maximum value of 96.08 % (with strain LY; GenBank accession no. U18747). This result indicates the necessity and propriety of a novel taxon for BCS phytoplasmas according to the recommendations of the IRPCM. Phylogenetic analysis was also conducted on 16S rRNA gene sequences, resulting in a monophyletic cluster composed of BCS-BoRand other LY-associated phytoplasmas. Other phytoplasmas on the island of New Guinea associated with banana wilt and arecanut yellow leaf diseases showed high similarities to BCS-BoRand were closely related to BCS phytoplasmas. Based on the uniqueness of their 16S rRNA gene sequences, a novel taxon ‘Ca.Phytoplasma noviguineense’ is proposed for these phytoplasmas found on the island of New Guinea, with strain BCS-BoR(GenBank accession no. LC228755) as the reference strain. The novel taxon is described in detail, including information on the symptoms of associated diseases and additional genetic features of thesecYgene andrpoperon.