Infrared MALDI mass spectrometry of large nucleic acids
Infrared MALDI mass spectrometry of large nucleic acids
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DOI：
10.1126/science.281.5374.260
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发表时间：
1998-07-10
期刊：
影响因子：
56.9
通讯作者：
Hillenkamp, F
中科院分区：
文献类型：
--
作者：
Berkenkamp, S;Kirpekar, F;Hillenkamp, F
质谱法已成为核酸常规分析的高准确度、高效率和高速度的日益重要的工具。为了使其可用于例如基因分型和临床诊断中所需的基因组材料的大规模测序，有必要找到允许分析比目前可能的100个核苷酸大得多的序列的方法。基质辅助激光解吸/电离（MALDI）质谱的合成DNA，质粒DNA的限制性内切酶片段，RNA转录的大小为2180个核苷酸的报告。1%或更高的质量准确度和几个飞摩尔或更少的样品要求超过了目前所有可用的大核酸分析技术。DNA和RNA的分析只需对样品纯化进行有限的投资。
Mass spectrometry has become an increasingly important tool of high accuracy, efficiency, and speed for the routine analysis of nucleic acids. To make it useful for large-scale sequencing of genomic material as required for example in genotyping and clinical diagnosis, it is necessary to find approaches that allow the analysis of sequences much larger than the 100 nucleotides currently possible. Matrix-assisted laser desorption/ionization (MALDI) mass spectra of synthetic DNA, restriction enzyme fragments of plasmid DNA, and RNA transcripts up to a size of 2180 nucleotides are reported. The demonstrated mass accuracy of 1 percent or better and the sample requirement of a few femtomoles or Less surpass all currently available techniques for the analysis of large nucleic acids. DNA and RNA can be analyzed with only a limited investment in sample purification.