DIFFERENTIAL EXPRESSION OF 5 N-METHYL-D-ASPARTATE RECEPTOR SUBUNIT MESSENGER-RNAS IN THE CEREBELLUM OF DEVELOPING-RATS AND ADULT-RATS
DIFFERENTIAL EXPRESSION OF 5 N-METHYL-D-ASPARTATE RECEPTOR SUBUNIT MESSENGER-RNAS IN THE CEREBELLUM OF DEVELOPING-RATS AND ADULT-RATS
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DOI：
10.1002/cne.903470112
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发表时间：
1994-09-01
影响因子：
2.5
通讯作者：
MIZUNO, N
中科院分区：
文献类型：
--
作者：
AKAZAWA, C;SHIGEMOTO, R;MIZUNO, N
目前已鉴定出5种N-甲基-D-天冬氨酸（NMDA）受体亚基：NR 1、NR 2A、NR 2B、NR 2C和NR 2D。在这里，我们分析了表达模式的NMDA受体亚基在发育和成年大鼠原位杂交。NR 1、NR 2A、NR 2B和NR 2C的mRNA在小脑外颗粒层中的表达在出生后3天出现杂交信号，而NR 2D的mRNA在所有发育阶段均不表达。NR 1 mRNA在所有发育阶段的小脑神经元中均有表达。NR 2A mRNA的信号出现在浦肯野细胞和颗粒细胞在出生后第二周。NR 2B mRNA在颗粒细胞中的信号在出生后的前2周内短暂可见。NR 2C mRNA的信号出现在颗粒细胞和胶质细胞在出生后第二周。NR 2D mRNA的信号出现短暂的浦肯野细胞在出生后的前8天，在成年大鼠，这些被认为是在星状细胞和高尔基体细胞。在小脑核中，NR 1，NR 2A，NR 2B和NR 2D的mRNA或多或少地在出生后0天表达，而NR 2C mRNA的表达信号在出生后14天首次检测到。因此，在出生后的前2周期间，在小脑皮质中观察到最明显的表达模式变化，此时小脑的发育和成熟进行得最快。(C)1994 Wiley-Liss，Inc.
Five N-methyl-D-aspartate (NMDA) receptor subunits have been identified thus far: NR1, NR2A, NR2B, NR2C, and NR2D. Here, we have analyzed the expression patterns of mRNAs for the NMDA receptor subunits in the developing and adult rats by in situ hybridization. The developmental changes of the expression patterns were most salient in the cerebellum.In the external granular layer, hybridization signals of mRNAs for NR1, NR2A, NR2B, and NR2C appeared by postnatal day 3, but no NR2D mRNA was expressed at any developmental stage examined. The NR1 mRNA was expressed in all cerebellar neurons at all developmental stages examined. The signals for the NR2A mRNA appeared in Purkinje cells and granule cells during the second postnatal week. The signals for the NR2B mRNA in granule cells were seen transiently during the first 2 weeks after birth. The signals for NR2C mRNA appeared in granule cells and glial cells during the second postnatal week. The signals for NR2D mRNA appeared transiently in Purkinje cells during the first 8 postnatal days; in adult rats, these were seen in stellate and Golgi cells. In the cerebellar nuclei, mRNAs for NR1, NR2A, NR2B, and NR2D were more or less expressed on postnatal day 0, while expression signals for the NR2C mRNA were first detected in postnatal day 14. Thus, the most conspicuous changes of expression patterns were observed in the cerebellar cortex during the first 2 weeks after birth, when development and maturation of the cerebellum proceed most rapidly. (C) 1994 Wiley-Liss, Inc.