Analysis of Neuropeptides in Stretched
Analysis of Neuropeptides in Stretched
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DOI：
10.1097/prs.0b013e3181a81542
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发表时间：
2009-07-01
影响因子：
3.6
通讯作者：
Ogawa, Rei
中科院分区：
文献类型：
--
作者：
Chin, Michael S.;Lancerotto, Luca;Ogawa, Rei
背景：机械力通过机械力转导调节创伤愈合和瘢痕形成。在响应机械刺激，神经肽从初级传入感觉神经元的外周末梢释放，影响皮肤和免疫细胞的功能，增加血管通透性，引起neurogenic inflammation.Methods：一个计算机控制的设备被用来拉伸小鼠皮肤。将C57 B16小鼠（n = 26）分配至4小时的周期性方波拉伸刺激或4小时的连续刺激。分析拉伸皮肤的神经肽、P物质和降钙素基因相关肽及其受体的表达（NK1R和降钙素基因相关肽受体组分蛋白）和生长因子（神经生长因子、转化生长因子β 1、血管内皮生长因子和表皮生长因子）使用免疫组织化学和实时逆转录聚合酶链反应。周期性刺激导致神经肽和生长因子的表达显著增加，而相应的肽受体下调。神经肽mRNA的转录在拉伸皮肤中升高，这证明神经肽不仅从神经纤维的外周末梢释放，而且还从驻留的皮肤cells.Conclusions：作者的结果表明，皮肤拉伸可能通过刺激神经肽表达而改变细胞生理学，并且周期性机械力可能更有效地刺激细胞上的机械敏感性伤害感受器或机械感受器（mechanosensors）。（Plast。重建124：102，2009）。
Background: Mechanical forces modulate wound healing and scar formation through mechanotransduction. In response to mechanical stimulation, neuropeptides are released from peripheral terminals of primary afferent sensory neurons, influencing skin and immune cell functions and increasing vascular permeability, causing neurogenic inflammation.Methods: A computer-controlled device was used to stretch murine skin. C57B16 mice (n = 26) were assigned to a cyclical square-wave tensile stimulation for 4 hours or continuous stimulation for 4 hours. Stretched skin was analyzed for expression of the neuropeptides, substance P and calcitonin gene-related peptide, their receptors (NK1R and calcitonin gene-related peptide receptor component protein), and growth factors (nerve growth factor, transforming growth factor beta 1, vascular endothelial growth factor, and epidermal growth factor) using immunohistochemistry and real-time reverse-transcriptase polymerase chain reaction.Results: Cyclical stimulation resulted in a significant increase in expression of neuropeptides and growth factors, whereas the corresponding peptide receptors were down-regulated. Transcription of neuropeptide mRNA was elevated in stretched skin, which proves that neuropeptides are released from not only peripheral terminals of nerve fibers but also resident skin cells.Conclusions: The authors' results suggest that skin stretching may alter cell physiology by stimulating neuropeptide expression, and that cyclical mechanical force may be more effectively stimulating mechanosensitive nociceptors or mechanoreceptors (mechanosensors) on cells. (Plast. Reconstr. Surg. 124: 102, 2009.)