Cell proliferation study on gastric carcinoma and non-involved gastric mucosa using a bromodeoxyuridine (BrdU) labelling technique.
Cell proliferation study on gastric carcinoma and non-involved gastric mucosa using a bromodeoxyuridine (BrdU) labelling technique.
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DOI：
10.1097/00008469-199210000-00006
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发表时间：
1992-10-01
期刊：
影响因子：
--
通讯作者：
Morris, R W
中科院分区：
文献类型：
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作者：
Brito, M J;Filipe, M I;Morris, R W
以前的报告使用[3 H]胸苷已显示改变模式的细胞增殖在胃癌发生。本研究应用溴脱氧尿苷（BrdU）体外摄取和免疫组化技术，对16例胃癌组织、癌旁组织和远处粘膜组织及10例正常对照进行了研究。计算了上、颈/峡部和下腺体隔室以及10个随机字段/癌的标记指数（LI）。癌组织的LI值高于癌旁或远粘膜及对照组（p < 0.001）。在非癌区域之间没有观察到差异。然而，LI在每个区域内的腺体隔室之间变化显著（p < 0.001）。在正常对照病例中，增殖细胞的位置仅限于颈部/峡部区域，并且可能最显著的观察结果是与远处粘膜和对照相比，邻近癌的正常粘膜中增殖区朝向表面的位置和扩展的变化（p < 0.001）。这些改变的细胞增殖模式可能是胃癌患者场变化的癌前病变潜力的一个重要方面，可以帮助患者随访，并作为监测干预研究的标志物。
Previous reports using [3H]thymidine have shown altered patterns of cell proliferation in gastric carcinogenesis. In this study we applied in vitro bromodeoxyuridine (BrdU) uptake and immunohistochemistry to tissue from carcinoma, its adjacent mucosa and distant mucosa (n = 16) cases and 10 normal control cases. The labelling index (LI) was calculated for the upper, neck/isthmus and lower gland compartments and in 10 random fields/carcinoma. The LI was greater in the carcinoma than in either the adjacent or far mucosa or the controls (p < 0.001). No difference was observed between the non-carcinoma areas. However, the LI varied significantly between the gland compartments within each area (p < 0.001). In the normal control cases, the location of the proliferating cells was limited to the neck/isthmus region and perhaps the most significant observation was the change of location and expansion of the proliferative zone towards the surface in the normal mucosa adjacent to carcinoma compared with distant mucosa and controls (p < 0.001). These altered patterns of cell proliferation may be an important aspect of the precancerous potential of field changes in gastric carcinoma patients and could help patient follow-up and serve as a marker in monitoring intervention studies.