Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin
Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin
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DOI：
10.1016/s0092-8674(00)80661-3
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发表时间：
1999-02-19
期刊：
影响因子：
64.5
通讯作者：
Stillman, B
中科院分区：
文献类型：
--
作者：
Shibahara, K;Stillman, B
染色质组装因子1（CAF-1）是表观遗传学决定的体内染色体状态的遗传所必需的，并且在体外DNA复制期间促进染色质的组装。在此，我们证明，DNA复制后，复制，但不是未复制的，DNA也是主管CAF-1依赖的染色质组装。增殖细胞核抗原（PCNA）是一种DNA聚合酶钳，是染色质组装DNA复制依赖性标记的组成部分。钳装载器，复制因子C（RFC），可以通过从复制的DNA中卸载PCNA来逆转该标记。PCNA直接与CAF-1的最大亚基p150结合，这两种蛋白质共定位于细胞中DNA复制的位点。我们认为PCNA和CAF-1将DNA复制与染色质组装和表观遗传染色体状态的遗传联系起来。
Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically determined chromosomal states in vivo and promotes assembly of chromatin during DNA replication in vitro. Herein, we demonstrate that after DNA replication, replicated, but not unreplicated, DNA is also competent for CAF-1-dependent chromatin assembly. The proliferating cell nuclear antigen (PCNA), a DNA polymerase clamp, is a component of the replication-dependent marking of DNA for chromatin assembly. The clamp loader, replication factor C (RFC), can reverse this mark by unloading PCNA from the replicated DNA. PCNA binds directly to p150, the largest subunit of CAF-1, and the two proteins colocalize at sites of DNA replication in cells. We suggest that PCNA and CAF-1 connect DNA replication to chromatin assembly and the inheritance of epigenetic chromosome states.