Evaluation of temperature effects on selectivity in RPLC separations using polybutadiene-coated zirconia.
Evaluation of temperature effects on selectivity in RPLC separations using polybutadiene-coated zirconia.
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DOI：
10.1021/ac9608681
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发表时间：
1997-06
影响因子：
7.4
通讯作者：
J. Li;P. Carr
中科院分区：
文献类型：
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作者：
J. Li;P. Carr
在聚丁二烯涂层氧化锆上评价了温度对RPLC方法发展中选择性的影响。我们发现，温度对选择性的影响强烈依赖于溶质类型。对于类似结构的溶质，如多环芳烃，温度对选择性几乎没有影响;然而，对于具有非常不同的官能团的溶质，如氯酚，温度变化确实显著影响选择性。我们认为具有一种主导保留机制的简单混合物-例如，疏溶剂保留-通过调节温度不会有明显的帮助。然而，在具有极性和可电离溶质的复杂混合物中，通过改变温度进行优化可能是富有成效的。
The effect of temperature on selectivity in RPLC method development has been evaluated on polybutadiene-coated zirconia. We find that the influence of temperature on selectivity depends strongly on solute type. For solutes of similar structure such as polyaromatic hydrocarbons, temperature has almost no effect on selectivity; however, for solutes with very different functional groups such as chlorophenols, temperature changes did significantly affect selectivity. We feel that simple mixtures with one dominant retention mechanism-e.g., solvophobic retention-will not be helped appreciably by adjusting temperature. However, in complex mixtures with polar and ionizable solutes, optimization by varying the temperature may well be fruitful.