TREPONEMA-DENTICOLA (EX BRUMPT 1925) SP-NOV, NOM REV, AND IDENTIFICATION OF NEW SPIROCHETE ISOLATES FROM PERIODONTAL POCKETS
TREPONEMA-DENTICOLA (EX BRUMPT 1925) SP-NOV, NOM REV, AND IDENTIFICATION OF NEW SPIROCHETE ISOLATES FROM PERIODONTAL POCKETS
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DOI：
10.1099/00207713-43-2-196
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发表时间：
1993-04-01
期刊：
影响因子：
--
通讯作者：
IUGOVAZ, I
中科院分区：
文献类型：
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作者：
CHAN, ECS;SIBOO, R;IUGOVAZ, I
使用标准生长和分离方法从深牙周袋中纯培养获得五种新的密螺旋体菌株。通过多种方法对菌株进行物种水平鉴定，包括凝集、免疫荧光、斑点印迹免疫测定、电子显微镜、代谢挥发性脂肪酸的气液色谱和DNA杂交。两个分离株是索氏密螺旋体 (Treponema socranskii) 菌株；另外三种是“齿垢密螺旋体”菌株，这是 Brumpt 于 1925 年描述的一个物种。由于之前没有为该物种指定模式菌株，因此该名称未包含在细菌名称批准列表中，并且目前没有命名法。我们建议齿垢密螺旋体 (Treponema denticola) (ex Brumpt) sp。十一月，名义。修订版是 Treponenta 属的有效且独特的物种，指定菌株 ATCC 35405 作为模式菌株，指定菌株 ATCC 33520 和 ATCC 35404 作为参考菌株。 T. denticola 似乎是最常从牙周袋中分离出来的物种。除非引入新的分离和培养技术，否则现有技术似乎只能产生属于当前描述的口腔厌氧螺旋体物种的分离株，并且分离较大密螺旋体的机会很小。
Standard growth and isolation methods were used to obtain five new treponema strains in pure culture from deep periodontal pockets. The strains were identified to the species level by various methods, including agglutination, immunofluorescence, a dot blot immunoassay, electron microscopy, gas-liquid chromatography of metabolic volatile fatty acids, and DNA hybridization. Two isolates were strains of Treponema socranskii; the other three were strains of ''Treponema denticola,'' a species described in 1925 by Brumpt. Because no type strain was designated for this species previously, the name was not included on the Approved Lists of Bacterial Names and has no current nomenclatural standing. We propose that Treponema denticola (ex Brumpt) sp. nov., nom. rev. is a valid and distinct species of the genus Treponenta and designate strain ATCC 35405 as the type strain and strains ATCC 33520 and ATCC 35404 as reference strains. T. denticola appears to be the species that is most frequently isolated from periodontal pockets. Unless new isolation and cultivation techniques are introduced, it appears that present technology can yield only isolates belonging to the currently described oral anaerobic spirochete species and that there is little chance of isolating the larger treponemes.