Determination of the crystal structure of EntA, a 2,3-dihydro-2,3-dihydroxybenzoic acid dehydrogenase from Escherichia coli.
Determination of the crystal structure of EntA, a 2,3-dihydro-2,3-dihydroxybenzoic acid dehydrogenase from Escherichia coli.
复制标题
确定 EntA（一种来自大肠杆菌的 2,3-二氢-2,3-二羟基苯甲酸脱氢酶）的晶体结构。
DOI：
10.1107/s0907444906015824
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Gulick,AndrewM
中科院分区：
文献类型：
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作者：
Sundlov,JesseA;Garringer,JulieA;Carney,JillM;Reger,AlbertS;Drake,EricJ;Duax,WilliamL;Gulick,AndrewM
大肠杆菌肠杆菌素合成簇由六种蛋白质EntA-EntF组成，它们由三个丝氨酸分子和三个2，3-二羟基苯甲酸（DHB）分子形成肠杆菌素分子。EntC、EntB和EntA催化由分支酸盐三步合成DHB。EntA是短链氧化还原酶（SCOR）蛋白家族的成员，催化DHB合成的最后一步，即2，3-二氢-2，3-二羟基苯甲酸向DHB的NAD+依赖性氧化。用多波长反常色散方法确定了EntA的结构。在这里，2.0纳米晶体结构的EntA在unliganded形式。 根据最近对SCOR家族其他成员的结构和生物信息学分析，结构分析提供了对参与辅因子和底物结合的残基的深入了解。
The Escherichia coli enterobactin synthetic cluster is composed of six proteins, EntA–EntF, that form the enterobactin molecule from three serine molecules and three molecules of 2,3-dihydroxybenzoic acid (DHB). EntC, EntB and EntA catalyze the three-step synthesis of DHB from chorismate. EntA is a member of the short-chain oxidoreductase (SCOR) family of proteins and catalyzes the final step in DHB synthesis, the NAD+-dependent oxidation of 2,3-dihydro-2,3-dihydroxybenzoic acid to DHB. The structure of EntA has been determined by multi-wavelength anomalous dispersion methods. Here, the 2.0 Å crystal structure of EntA in the unliganded form is presented. Analysis of the structure in light of recent structural and bioinformatic analysis of other members of the SCOR family provides insight into the residues involved in cofactor and substrate binding.