Evolutionary linkage between eukaryotic cytokinesis and chloroplast division by dynamin proteins
Evolutionary linkage between eukaryotic cytokinesis and chloroplast division by dynamin proteins
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DOI：
10.1073/pnas.0802412105
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发表时间：
2008-09-30
影响因子：
11.1
通讯作者：
Nakanishi, Hiromitsu
中科院分区：
文献类型：
--
作者：
Miyagishima, Shin-ya;Kuwayama, Hidekazu;Nakanishi, Hiromitsu
叶绿体是由蓝藻内共生体进化而来的，在真核细胞繁殖过程中通过叶绿体的协调分裂而被保留了10多亿年。叶绿体的分裂是由分裂部位的环结构完成的，包括两个被膜的内部和外部。分裂机制的一部分来自蓝藻基于FtsZ蛋白的细胞动力学活性。相反，分裂机制的其他部分涉及真核生物特有的蛋白质，包括动力蛋白家族的一个成员。动力蛋白家族的每个成员都参与了一个不同的真核膜系统的分裂或融合。为了深入了解进化来调节叶绿体分裂的祖先动力蛋白和真核膜活性，我们研究了与叶绿体分裂蛋白关系最密切的动力蛋白的功能。这些蛋白质在盘状网囊阿米巴和拟南芥中定位于细胞分裂部位，参与胞质分裂。我们的结果表明，叶绿体分裂的动力蛋白来源于参与真核细胞胞质分裂的动力蛋白。因此，叶绿体分裂机制是细菌胞质分裂和真核胞质分裂的混合体，而真核胞质分裂是内共生细胞分裂与寄主细胞分裂同步的关键因素，从而有助于建立持久的内共生关系。
Chloroplasts have evolved from a cyanobacterial endosymbiont and been retained for more than 1 billion years by coordinated chloroplast division in multiplying eukaryotic cells. Chloroplast division is performed by ring structures at the division site, encompassing both the inside and the outside of the two envelopes. A part of the division machinery is derived from the cyanobacterial cytokinetic activity based on the FtsZ protein. In contrast, other parts of the division machinery involve proteins specific to eukaryotes, including a member of the dynamin family. Each member of the dynamin family is involved in the division or fusion of a distinct eukaryotic membrane system. To gain insight into the kind of ancestral dynamin protein and eukaryotic membrane activity that evolved to regulate chloroplast division, we investigated the functions of the dynamin proteins that are most closely related to chloroplast division proteins. These proteins in the amoeba Dictyostelium discoideum and Arabidopsis thaliana localize at the sites of cell division, where they are involved in cytokinesis. Our results suggest that the dynamin for chloroplast division is derived from that involved in eukaryotic cytokinesis. Therefore, the chloroplast division machinery is a mixture of bacterial and eukaryotic cytokinesis components, with the latter a key factor in the synchronization of endosymbiotic cell division with host cell division, thus helping to establish the permanent endosymbiotic relationship.