N-acetyltransferase Mpr1 confers freeze tolerance on Saccharomyces cerevisiae by reducing reactive oxygen species
N-acetyltransferase Mpr1 confers freeze tolerance on Saccharomyces cerevisiae by reducing reactive oxygen species
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DOI：
10.1093/jb/mvi134
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发表时间：
2005-10-01
影响因子：
2.7
通讯作者：
Takagi, H
中科院分区：
文献类型：
--
作者：
Du, XY;Takagi, H
酿酒酵母N-乙酰转移酶Mpr1能降低细胞内氧化水平，保护氧化应激状态下的酵母细胞。我们发现，酵母细胞在冷冻和解冻过程中表现出更高的活性氧水平。基因中断和表达实验表明，Mpr1通过降低细胞内活性氧水平来保护酵母细胞免受冷冻应激的影响。Mpr1与L-脯氨酸联合使用可进一步增强其抗冻胁迫能力。因此，Mpr1和L-Pro在选育耐冻酵母新菌株方面具有广阔的应用前景。
N-Acetyltransferase Mpr1 of Saccharomyces cerevisiae can reduce intracellular oxidation levels and protect yeast cells under oxidative stress. We found that yeast cells exhibited increased levels of reactive oxygen species during freezing and thawing. Gene disruption and expression experiments indicated that Mpr1 protects yeast cells from freezing stress by reducing the intracellular levels of reactive oxygen species. The combination of Mpr1 and L-proline could further enhance the resistance to freezing stress. Hence, Mpr1 as well as L-proline has promising potential for the breeding of novel freeze-tolerant yeast strains.