Development of an enzymatic reaction device using magnetic bead-cluster handling
Development of an enzymatic reaction device using magnetic bead-cluster handling
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DOI：
10.1088/0960-1317/16/9/017
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发表时间：
2006-09-01
影响因子：
2.3
通讯作者：
Sato, Kazuo
中科院分区：
文献类型：
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作者：
Shikida, Mitsuhiro;Takayanagi, Kentaro;Sato, Kazuo
我们先前提出了一种用于微全分析系统（Micro-TAS）的磁珠团处理装置，并研究了其工作原理。该装置不需要机械流体装置，如泵和阀，用于处理溶液。我们进一步开发了生化反应单元芯片，这是一个关键组成部分，在微型TAS，通过应用珠簇处理机制。我们能够通过使用碱性磷酸酶和对硝基苯基磷酸酯之间的水解来进行酶促反应。我们还证实，所获得的校准曲线在酶促反应期间呈线性。通过进行对比实验，我们在反应芯片上具有70%的反应效率。从这些结果中，我们得出结论，我们开发的反应芯片是适用于酶免疫测定系统。
We previously proposed a magnetic bead-cluster handling device for Micro-total analysis systems (Micro-TAS) and investigated its operation principle. The device does not need mechanical fluidic devices, such as pumps and valves, for handling solutions. We further developed the biochemical reaction unit chip, which is a key component in Micro-TAS, by applying a bead-cluster handling mechanism. We were able to do the enzymatic reaction by using hydrolysis between alkaline phosphatase and p-nitrophenyl phosphate. We also confirmed that the obtained calibration curves were linear during the enzymatic reaction. We had 70% reaction efficiency on the reaction chip by performing a comparative experiment. From these results, we concluded that our developed reaction chip is applicable to enzymatic immuno-assay systems.