Tetrahymena in the laboratory: strain resources, methods for culture, maintenance, and storage.
Tetrahymena in the laboratory: strain resources, methods for culture, maintenance, and storage.
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DOI：
10.1016/b978-0-12-385967-9.00008-6
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发表时间：
2012
影响因子：
--
通讯作者：
Cassidy-Hanley, Donna M.
中科院分区：
文献类型：
--
作者：
Cassidy-Hanley, Donna M.
四膜虫是一种重要的生物学研究模式动物。在此期间，已经鉴定了多种有用的菌株，包括高度近交的原种、多种突变菌株的集合以及来自各种地理位置的野生栽培种。此外，由于许多不同实验室的努力，四膜虫的生长，维持和储存的最佳条件已经制定出来。为了促进四膜虫的有效利用，特别是那些新的系统，本章提出了许多可用的四膜虫菌株的简要说明，并列出了可能的资源，以获得可行的培养T。thermophila和其他Tetrahymena物种。还介绍了常用培养基、细胞培养和维护方法以及短期和长期储存方案。
The ciliated protozoan Tetrahymena thermophila has been an important model system for biological research for many years. During that time a variety of useful strains, including highly inbred stocks, a collection of diverse mutant strains, and wild cultivars from a variety of geographical locations have been identified. In addition, thanks to the efforts of many different laboratories, optimal conditions for growth, maintenance, and storage of Tetrahymena have been worked out. To facilitate the efficient use of Tetrahymena, especially by those new to the system, this chapter presents a brief description of many available Tetrahymena strains, and lists possible resources for obtaining viable cultures of T. thermophila and other Tetrahymena species. Descriptions of commonly used media, methods for cell culture and maintenance, and protocols for short and long term storage are also presented.