ISOLATION AND DNA-SEQUENCE OF A CDNA CLONE ENCODING A LYMPHOCYTE ADHESION RECEPTOR FOR HIGH ENDOTHELIUM
ISOLATION AND DNA-SEQUENCE OF A CDNA CLONE ENCODING A LYMPHOCYTE ADHESION RECEPTOR FOR HIGH ENDOTHELIUM
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DOI：
10.1073/pnas.86.12.4659
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发表时间：
1989-06-01
影响因子：
11.1
通讯作者：
STJOHN, T
中科院分区：
文献类型：
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作者：
IDZERDA, RL;CARTER, WG;STJOHN, T
淋巴细胞从血流中迁移到次级淋巴器官，这是成功免疫应答所必需的，主要发生在以高壁内皮细胞为特征的毛细血管后小静脉内。淋巴细胞在这些部位的粘附和外渗与这种专门的小静脉内皮细胞的特异性淋巴样受体的表达有关。我们在这里报告的分子克隆从狒狒淋巴细胞系的cDNA编码的粘附受体戊型肝炎病毒。由该cDNA编码的362个氨基酸的蛋白质不存在于所检查的任何数据库中。成熟的蛋白质，从一个假定的20个氨基酸的信号肽的切割，具有计算的分子量只有37 kDa，表明90 kDa的细胞表面蛋白是高度修饰。342个氨基酸，其缺乏任何报道的显著长度的序列，包括胞外结构域（250个氨基酸）、推定的跨膜结构域（20个氨基酸）和胞质结构域（72个氨基酸）。
The migration of lymphocytes from the bloodstream into the secondary lymphoid organs, which is necessary for a successful immune response, occurs primarily within postcapillary venules that are characterized by high-walled endothelial cells. Lymphocytes adhesion to and extravasation at these sites is associated with the expression of specific lymphoid receptors for this specialized venule endothelium. We report here the molecular cloning from a baboon lymphoid cell line of a cDNA that encodes an adhesion receptor for HEV. The 362-amino acid protein encoded by this cDNA is not present in any of the data bases examined. The mature protein, resulting from the cleavage of a putative 20-amino acid signal peptide, has a calculated molecular mass of only 37 kDa, indicating that the 90-kDa cell surface protein is highly modified. The 342 amino acids, which lack any reported sequences of significant length, encompass an extracellular domain (250 amino acids), a putative transmembrane domain (20 amino acids), and a cytoplasmic domain (72 amino acids).