A reinvestigation of the lipopolysaccharide structure of Helicobacter pylori strain Sydney (SS1)
A reinvestigation of the lipopolysaccharide structure of Helicobacter pylori strain Sydney (SS1)
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DOI：
10.1111/j.1742-4658.2011.08270.x
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发表时间：
2011-09-01
期刊：
影响因子：
5.4
通讯作者：
Vinogradov, Evgeny
中科院分区：
文献类型：
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作者：
Altman, Eleonora;Chandan, Vandana;Vinogradov, Evgeny
在这项研究中，我们描述了一个重新调查的脂多糖（LPS）结构的幽门螺杆菌菌株悉尼（SS 1）的基础上获得的低聚糖的NMR分析，通过使用各种降解的LPS以及毛细管电泳-MS数据。分析结果表明，H. pylori SS 1 LPS糖型由D-甘油-D-甘露庚糖（DD-Hep）残基被直链取代的骨架核心寡糖组成，直链由三糖片段α-DDHep-3-α-L-Fuc-3-β-GlcNAc组成，如先前对H. pylori菌株26695，通过连续添加α-Glc和β-Gal残基进一步延长，并终止于由1，2-连接的β-呋喃核糖基残基组成的新的线性链，其中最后的β-呋喃核糖基残基提供O-链多糖的连接点：α-Glc-4-β-Gal-7-α-DDHep-2-α-Hep-3-α-Hep7PEtn-5-α-Kdo-6-β-GlcN-6-α-GlcN1PEtn竖条R（2）R=PS-2-β-Ribf-[2-β-Ribf-]（n）-4-β-Gal-3-α-Glc-7-α-DDHep-3-α-L-Fuc-3-β-GlcN-]（n）是1，2-连接的β-呋喃核糖（ribofuranose，riban）的短（3 - 5个残基）寡聚体，PS是由N-乙酰基乳糖胺组成的多糖链，被α-Fuc取代形成刘易斯（Le）型结构。除了先前鉴定的LacNAc、Le（y）和Le（x）组分外，H.发现pylori SS 1 LPS含有一个新的LacNAc单元，该单元在β-GlcNAc残基的O-6位携带磷酸乙醇胺取代基。
In this study, we describe a reinvestigation of the lipopolysaccharide (LPS) structure of Helicobacter pylori strain Sydney (SS1) based on the NMR analysis of oligosaccharides obtained through the use of various degradations of the LPS as well as capillary electrophoresis-MS data. The results of the analysis indicated that the core region of a major H. pylori SS1 LPS glycoform consists of a backbone core oligosaccharide substituted at the D-glycero-D-manno-heptose (DD-Hep) residue by a linear chain composed of a trisaccharide fragment alpha-DDHep-3-alpha-L-Fuc-3-beta-GlcNAc, as previously demonstrated for H. pylori strain 26695, further elongated by consecutively added alpha-Glc and beta-Gal residues, and terminating in a novel linear chain consisting of 1,2-linked beta-ribofuranosyl residues, where the last beta-ribofuranosyl residue provides a point of attachment for the O-chain polysaccharide:alpha-Glc-4-beta-Gal-7-alpha-DDHep-2-alpha-Hep-3-alpha-Hep7PEtn-5-alpha-Kdo-6-beta-GlcN-6-alpha-GlcN1PEtnvertical bar R(2)R=PS-2-beta-Ribf-[2-beta-Ribf-](n)-4-beta-Gal-3-alpha-Glc-7-alpha-DDHep-3-alpha-L-Fuc-3-beta-GlcN-where [2-beta-Ribf-](n) is a short (three to five residues) oligomer of 1,2-linked beta-ribofuranose (riban), and PS is a polysaccharide chain consisting of N-acetyllactosamine, substituted with alpha-Fuc to form Lewis (Le)-type structures. In addition to the previously identified LacNAc, Le(y) and Le(x) components, the O-chain polysaccharide of H. pylori SS1 LPS was found to contain a novel LacNAc unit carrying a phosphoethanolamine substituent at the O-6 position of beta-GlcNAc residues.