Structural features of collapsin required for biological activity and distribution of binding sites in the developing chick
Structural features of collapsin required for biological activity and distribution of binding sites in the developing chick
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DOI：
10.1006/mcne.1997.0636
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发表时间：
1997-01-01
影响因子：
3.5
通讯作者：
Doherty, P
中科院分区：
文献类型：
--
作者：
Eickholt, BJ;Morrow, R;Doherty, P
我们已经使用Fc-嵌合体的reglosin-1/Sema III的结构-功能活性的研究，最近确定的排斥性轴突导向分子，并映射鸡发育过程中的分布的IFS结合位点。我们的研究结果表明，在体外塌陷测定中的生物学活性的白藜芦醇-Fc是独立的Ig结构域和带正电荷的羧基末端。在一些神经纤维束上发现了塌陷蛋白结合位点，并且在两种情况下（DRG束和refinotectal投射），这种表达是高度动态的，并且与它们在轴突生长和指导中发挥作用一致。Collapsin-1结合位点也被发现在一些非神经元结构，不产生collapsin-1 mRNA。我们假设，这些网站可能会采取行动，本地化或集中从不断增长的轴突中释放，并以这种方式允许自分泌轴突的指导机制？在发展过程中。
We have used Fc-chimeras of collapsin-1/Sema III to study the structure-function activity of this recently identified repulsive axonal guidance molecule and to map the distribution of ifs binding sites during chick development. Our results show that the biological activity of the collapsin-Fc in an in vitro collapse assay is independent of both the Ig-domain and the positive charged carboxy terminus. Collapsin binding sites were found on a number of neuronal fiber tracts, and in two instances (DRG tracts and the refinotectal projection) this expression is both highly dynamic and consistent with them playing a role in axonal growth and guidance. Collapsin-1 binding sites were also found on a number of nonneuronal structures that do not produce collapsin-1 mRNA. We postulate that these sites may act to localize or concentrate collapsin-1 released from growing axons and in this way allow for an autocrine axonal guidance mechanism to ?unction during development.