Genetic Population Structure of Chum Salmon in the Pacific Rim Inferred from Mitochondrial DNA Sequence Variation
Genetic Population Structure of Chum Salmon in the Pacific Rim Inferred from Mitochondrial DNA Sequence Variation
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DOI：
10.1023/b:ebfi.0000022881.90237.aa
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发表时间：
2004-03
影响因子：
1.4
通讯作者：
S. Sato;H. Kojima;J. Ando;H. Ando;R. Wilmot;L. Seeb;V. Efremov;L. Leclair;W. Buchholz;D. Jin;S. Urawa;M. Kaeriyama;A. Urano;S. Abe
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作者：
S. Sato;H. Kojima;J. Ando;H. Ando;R. Wilmot;L. Seeb;V. Efremov;L. Leclair;W. Buchholz;D. Jin;S. Urawa;M. Kaeriyama;A. Urano;S. Abe
我们研究了鲑鱼，虹鳟鱼，在环太平洋地区使用线粒体（mt）DNA分析的遗传种群结构。对mtDNA控制区5′端可变区约500 bp的核苷酸序列进行分析，发现20个核苷酸可变位点，可确定3个进化枝的30种单倍型（A，B和C），来自日本（16），韩国（1），俄罗斯（10）和北美的48个种群的2，100多个个体（21人来自阿拉斯加、不列颠哥伦比亚省和华盛顿）。观察到的单倍型大多与地理区域，在分支A和C单倍型的特点亚洲人口和分支B单倍型区分北美人口。单倍型的多样性是最高的，在日本的人口，这表明更大的遗传变异，在日本的人口比俄罗斯和北美。分子方差分析和列联χ 2检验表明，三个地理群体之间的结构性较强，而日本和北美群体内的结构性较弱至中等。这些结果表明，所观察到的地理格局可能主要受历史扩张或殖民化的影响，其次是区域内当地群体之间的低或有限的基因流。除了种群结构的分析，mtDNA数据可能是有用的，为建立一个基线的公海鲑鱼混合种群的股票识别。
We examined the genetic population structure of chum salmon,Oncorhynchus keta, in the Pacific Rim using mitochondrial (mt) DNA analysis. Nucleotide sequence analysis of about 500 bp in the variable portion of the 5′ end of the mtDNA control region revealed 20 variable nucleotide sites, which defined 30 haplotypes of three genealogical clades (A, B, and C), in more than 2,100 individuals of 48 populations from Japan (16), Korea (1), Russia (10), and North America (21 from Alaska, British Columbia, and Washington). The observed haplotypes were mostly associated with geographic regions, in that clade A and C haplotypes characterized Asian populations and clade B haplotypes distinguished North American populations. The haplotype diversity was highest in the Japanese populations, suggesting a greater genetic variation in the populations of Japan than those of Russia and North America. The analysis of molecular variance and contingency χ2tests demonstrated strong structuring among the three geographic groups of populations and weak to moderate structuring within Japanese and North American populations. These results suggest that the observed geographic pattern might be influenced primarily by historic expansions or colonizations and secondarily by low or restricted gene flow between local groups within regions. In addition to the analysis of population structure, mtDNA data may be useful for constructing a baseline for stock identification of mixed populations of high seas chum salmon.