In Vivo Two-Photon Imaging of Microglial Synapse Contacts
In Vivo Two-Photon Imaging of Microglial Synapse Contacts
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小胶质细胞突触接触的体内双光子成像
DOI：
10.1007/978-1-4939-9658-2_20
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Wake Hiroaki
中科院分区：
文献类型：
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作者：
Kato Daisuke;Ikegami Ako;Horiuchi Hiroshi;Moorhouse Andrew J.;Nabekura Junichi;Wake Hiroaki
小胶质细胞传统上被认为是大脑的免疫哨兵，并且在神经退行性疾病如阿尔茨海默病、帕金森病或肌萎缩侧索硬化症的发病机制中起关键作用。最近，它们也被确定为突触组织者，促进突触的形成和成熟以及调节突触活动。有趣的是，小胶质细胞介导的突触修剪和小胶质细胞介导的突触可塑性的变化在大脑发育和神经退行性疾病中均被观察到，强调了小胶质细胞-突触相互作用在这些过程中的关键作用。在这里，我们描述了一种通过双光子显微镜在体内无创监测小胶质细胞-突触相互作用的技术。
Microglia are traditionally known as immune sentinels of the brain and as key player in the pathogenesis of neurodegenerative diseases such as Alzheimer’s disease, Parkinson disease, or amyotrophic lateral sclerosis. Recently, they were also identified as synaptic organizer, promoting formation and maturation of synapses as well as modifying synaptic activity. Interestingly, microglia-mediated synaptic pruning and microglia-mediated changes in synaptic plasticity were observed both during brain development and in neurodegenerative diseases, stressing the key role of microglia-synapse interaction in these processes. Here we descried a technique for noninvasive in vivo monitoring of microglia-synapse interactions by means of two-photon microscopy.