Monocyte and macrophage activation by lipoteichoic acid is independent of alanine and is potentiated by hemoglobin
Monocyte and macrophage activation by lipoteichoic acid is independent of alanine and is potentiated by hemoglobin
复制标题
DOI：
10.4049/jimmunol.176.9.5567
复制
发表时间：
2006-05-01
影响因子：
4.4
通讯作者：
Ofek, Itzhak
中科院分区：
文献类型：
--
作者：
Hasty, David L.;Meron-Sudai, Shiri;Ofek, Itzhak
脂磷壁酸（LTA）是革兰氏阳性细菌细胞壁成分，可引起单核细胞分泌细胞因子。细胞因子刺激活性被认为依赖于沿着聚甘油磷酸骨架保留高水平的酯连接的D-丙氨酸残基沿着。然而，基本上不含D-丙氨酸的化脓性链球菌LTA引起人和小鼠细胞分泌与具有高得多的D-丙氨酸含量的LTA一样多的IL-6。此外，血红蛋白（Hb）显着增强各种LTA对小鼠巨噬细胞或人血细胞的刺激作用，无论其D-丙氨酸含量。LTA和Hb似乎形成一种分子复合物，这是基于各自影响另一个在天然丙烯酰胺凝胶上的迁移的能力、它们在这些凝胶上的共迁移以及LTA改变Hb的吸收光谱的能力。因为S.虽然已知化脓性链球菌释放LTA并分泌至少两种有效的溶血毒素，但LTA-Hb相互作用可在链球菌感染期间发生，并可能导致局部炎症反应的显著改变。
Lipoteichoic acids (LTAs) are Gram-positive bacterial cell wall components that elicit mononuclear cell cytokine secretion. Cytokine-stimulating activity is thought to be dependent on retaining a high level of ester-linked D-alanine residues along the polyglycerol phosphate backbone. However, Streptococcus pyogenes LTA essentially devoid Of D-alanine caused human and mouse cells to secrete as much IL-6 as LTA with a much higher D-alanine content. Furthermore, hemoglobin (Hb) markedly potentiates the stimulatory effect of various LTAs on mouse macrophages or human blood cells, regardless of their D-alanine content. LTA and Hb appear to form a molecular complex, based on the ability of each to affect the other's migration on native acrylamide gels, their comigration on these gels, and the ability of LTA to alter the absorption spectra of Hb. Because S. pyogenes is known to release LTA and secrete at least two potent hemolytic toxins, LTA-Hb interactions could occur during streptococcal infections and might result in a profound alteration of the local inflammatory response.