Follicular Helper T Cell Derived Exosomes Promote B Cell Proliferation and Differentiation in Antibody-Mediated Rejection after Renal Transplantation
Follicular Helper T Cell Derived Exosomes Promote B Cell Proliferation and Differentiation in Antibody-Mediated Rejection after Renal Transplantation
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肾移植后抗体介导的排斥反应中滤泡辅助 T 细胞衍生的外泌体促进 B 细胞增殖和分化
DOI：
10.1155/2019/6387924
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发表时间：
2019
影响因子：
--
通讯作者：
Shi Bingyi
中科院分区：
文献类型：
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作者：
Yang Jintao;Bi Lili;He Xiuyun;Wang Zhen;Qian Yeyong;Xiao Li;Shi Bingyi
滤泡辅助性T细胞(TFH细胞)与肾移植后抗体介导的排斥反应(AMR)的发生和发展密切相关。外切体在器官移植后排斥反应中起关键作用。然而，TFH来源的外切体是否参与AMR尚未见报道。采集42例肾移植患者外周血，发现AMR组与非AMR组患者外周血中的CD4+CXCR5+和CD4+CXCR5+CXCR3+CCR6-外显体的比例无显著差异，而AMR组的CD4+CXCR5+CXCR3-外显体的比例明显高于非AMR组，而其外显体CTLA-4的表达明显低于非AMR组。两组间HL A-G表达差异无统计学意义。我们用磁珠进一步分离了患者体内的CD4+CXCR5+细胞。共培养实验显示，急性髓细胞白血病患者TFH细胞来源的外切体明显促进B细胞的增殖和分化，与非急性髓细胞白血病患者相比，B细胞和浆细胞比例分别增加87.52%和110.2%。综上所述，我们的研究发现，TFH细胞来源的外切体能够促进B细胞的增殖和分化，可能在肾移植后AMR的发生发展中起重要作用。
Follicular helper T cells (Tfh cells) are closely related to the occurrence and development of antibody‐mediated rejection (AMR) after renal transplantation. Exosomes play a key role in the rejection after organ transplantation. However, whether Tfh‐derived exosomes are involved in AMR has not been reported. We collected peripheral blood from 42 kidney transplant patients and found no significant differences in CD4+CXCR5+ and CD4+CXCR5+CXCR3+CCR6‐exosomes between AMR and non‐AMR groups, whereas the proportion of CD4+CXCR5+CXCR3‐exosomes was significantly higher in AMR group than that in non‐AMR group; CTLA‐4 expression of CD4+CXCR5+exosomes was significantly lower in AMR group than that in non‐AMR group. HLA‐G expression was not significantly different between two groups. We further separated CD4+CXCR5+cells from patients by magnetic beads. Coculture experiments showed that Tfh cell‐derived exosomes in AMR patients significantly promoted B cell proliferation and differentiation, compared with non‐AMR group, the percentage of B cells and plasma cells increased by 87.52% and 110.2%, respectively. In conclusion, our study found that Tfh cell‐derived exosomes could promote the proliferation and differentiation of B cells and they may play an important role in the development of AMR after renal transplantation.