cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma.
cDNA clones encoding IgE-binding factors from a rat-mouse T-cell hybridoma.
复制标题
编码来自大鼠-小鼠 T 细胞杂交瘤的 IgE 结合因子的 cDNA 克隆。
DOI：
10.1073/pnas.82.8.2460
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Moore,KW
中科院分区：
文献类型：
--
作者：
Martens,CL;Huff,TF;Jardieu,P;Trounstine,ML;Coffman,RL;Ishizaka,K;Moore,KW
从分泌IgE抑制因子（IgE- sf）的大鼠-小鼠T杂交瘤cDNA文库中分离到编码啮齿动物IgE结合因子（IgE- bf）的cDNA克隆。转染了其中两种cdna的COS7细胞表达IgE- bf，选择性地增强了体外IgE反应。在COS7细胞中表达的IgE-BF是约等于60和约等于11 kDa的糖蛋白。对IgE-BF cDNA进行序列分析，发现一个556个氨基酸（62 kDa）的蛋白质编码区。结果表明，ige增强因子和ige抑制因子具有共同的前体多肽，11kda的IgE-BF来源于60kda的前体。
cDNA clones encoding rodent IgE-binding factors (IgE-BF) were isolated from cDNA libraries of a rat-mouse T hybridoma that secretes IgE-suppressive factor (IgE-SF) upon incubation with rat IgE. COS7 cells transfected with two of the cDNAs expressed IgE-BF, which selectively potentiate an in vitro IgE response. IgE-BF expressed in COS7 cells are glycoproteins of approximately equal to 60 and approximately equal to 11 kDa. DNA sequence analysis of an IgE-BF cDNA revealed a 556-amino acid (62 kDa) protein coding region. The results suggest that IgE-potentiating and IgE-suppressive factors share common precursor polypeptides and that the 11-kDa IgE-BF is derived from a 60-kDa precursor.