FUNDAMENTALS OF QUANTITATIVE AUTORADIOGRAPHY BY COMPUTER DENSITOMETRY FOR IN-SITU HYBRIDIZATION, WITH EMPHASIS ON P-33
FUNDAMENTALS OF QUANTITATIVE AUTORADIOGRAPHY BY COMPUTER DENSITOMETRY FOR IN-SITU HYBRIDIZATION, WITH EMPHASIS ON P-33
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DOI：
10.1177/41.12.8245425
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发表时间：
1993-12-01
影响因子：
3.2
通讯作者：
STAHL, WL
中科院分区：
文献类型：
--
作者：
BASKIN, DG;STAHL, WL
原位杂交（ISH）中的定量放射自显影（QAR）的目的是确定产生放射自显影图像的组织切片的相应区域中存在的放射性寡核苷酸或核糖核酸探针的量。本文讨论了（a）为实现这一目标而选择和使用计算机图象分析系统的有关考虑，（B）用P-33研制ISH QAR的C-14塑料放射自显影标准，（c）用QAR研制ISH探头的T-m曲线，（d）用视频成象系统测量QAR的分辨率。
The goal of quantitative autoradiography (QAR) in in situ hybridization (ISH) is to determine the amount of radioactive oligonucleotide or riboprobe present in the corresponding area of the tissue slice that produced an autoradiographic image. This article discusses (a) some of the considerations related to selection and use of computer image analysis systems for accomplishing this objective, (b) development of C-14 plastic autoradiographic standards for ISH QAR with P-33, (c), using QAR to develop T-m curves for ISH probes, and (d) measurement of resolution with video imaging systems for QAR.