A role for the acetylcholine receptor-inducing protein ARIA in oligodendrocyte development.
A role for the acetylcholine receptor-inducing protein ARIA in oligodendrocyte development.
复制标题
乙酰胆碱受体诱导蛋白 ARIA 在少突胶质细胞发育中的作用。
DOI：
10.1073/pnas.91.24.11626
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发表时间：
1994
影响因子：
11.1
通讯作者：
K. Stefansson
中科院分区：
文献类型：
--
作者：
T. Vartanian;G. Corfas;Y. Li;G. Fischbach;K. Stefansson
ARIA乙酰胆碱受体诱导活性蛋白，是一个配体家族的成员，包括新分化因子，heregulin和胶质生长因子。这些配体都通过一种或多种约185 kDa的酪氨酸激酶受体起作用。在某些情况下，这些配体促进增殖，而在另一些情况下，它们诱导分化。ARIA最初是从鸡脑中分离出来的，它能够诱导骨骼肌中烟碱乙酰胆碱受体的合成。在本文中，我们发现ARIA在大鼠脑室下区表达，并促进双电位（O2A）胶质祖细胞向少突胶质细胞的发育。我们还发现ARIA诱导O2A祖细胞中185 kda蛋白的酪氨酸磷酸化。ARIA不会增加少突胶质细胞中溴脱氧尿苷的掺入，但当添加到体外雪旺细胞中时，会产生有丝分裂。因此，ARIA在体外加速了少突胶质细胞的形成，并在体内表达它可以发挥同样的影响。
ARIA acetylcholine receptor-inducing activity protein, is a member of a family of ligands that includes the Neu differentiation factor, heregulin, and glial growth factor. These ligands all act through one or more receptor tyrosine kinases of approximately 185 kDa. In some conditions these ligands promote proliferation, whereas in others they induce differentiation. ARIA was originally isolated from chick brain on the basis of its ability to induce synthesis of nicotinic acetylcholine receptors in skeletal muscle. In this paper we show that ARIA is expressed in the subventricular zone of the rat brain and that it enhances the development of oligodendrocytes from bipotential (O2A) glial progenitor cells. We have also found that ARIA induces tyrosine phosphorylation of a 185-kDa protein in O2A progenitor cells. ARIA does not increase bromodeoxyuridine incorporation by oligodendrocytes but is mitogenic when added to Schwann cells in vitro. Thus, ARIA accelerates the formation of oligodendrocytes in vitro and is expressed where it could exercise the same influence in vivo.