Three-dimensional secretion signals in chaperone-effector complexes of bacterial pathogens
Three-dimensional secretion signals in chaperone-effector complexes of bacterial pathogens
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DOI：
10.1016/s1097-2765(02)00529-4
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发表时间：
2002-05-01
期刊：
影响因子：
16
通讯作者：
Ghosh, P
中科院分区：
文献类型：
--
作者：
Birtalan, SC;Phillips, RM;Ghosh, P
革兰氏阴性细菌病原体的III型分泌系统（TTSS）将毒力所需的效应蛋白直接递送到宿主细胞的胞质溶胶中。许多效应子的传递依赖于与细菌胞质溶胶中特定同源分子伴侣的结合。分子伴侣的作用机制尚不清楚。在这里，我们提出的耶尔森氏菌SycE-YopE分子伴侣效应复合物的生物化学和晶体学结果，与以前的模型相矛盾的分子伴侣功能，并证明分子伴侣的作用是孤立的，只有一小部分的效应。这一点，连同证据的立体化学保守分子伴侣效应复合物，否则是不相关的序列，表明这些复合物的功能一般，三维TTSS分泌信号，并可能赋予一个时间顺序分泌。
The type III secretion system (TTSS) of Gram-negative bacterial pathogens delivers effector proteins required for virulence directly into the cytosol of host cells. Delivery of many effectors depends on association with specific cognate chaperones in the bacterial cytosol. The mechanism of chaperone action is not understood. Here we present biochemical and crystallographic results on the Yersinia SycE-YopE chaperone-effector complex that contradict previous models of chaperone function and demonstrate that chaperone action is isolated to only a small portion of the effector. This, together with evidence for stereochemical conservation between chaperone-effector complexes, which are otherwise unrelated in sequence, indicates that these complexes function as general, three-dimensional TTSS secretion signals and may endow a temporal order to secretion.