Piezoelectric immunosensor for bisphenol A based on signal enhancing step with 2-methacrolyloxyethyl phosphorylcholine polymeric nanoparticle
Piezoelectric immunosensor for bisphenol A based on signal enhancing step with 2-methacrolyloxyethyl phosphorylcholine polymeric nanoparticle
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DOI：
10.1039/b511662g
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发表时间：
2006-01-01
期刊：
影响因子：
4.2
通讯作者：
Ishihara, K
中科院分区：
文献类型：
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作者：
Park, JW;Kurosawa, S;Ishihara, K
BPA与BPA-辣根过氧化物酶结合物竞争结合抗BPA抗体的免疫测定法，与压电（PZ）免疫传感器偶联，能够检测到0.1 ng mL（-1）BPA。为了提高检测的灵敏度，我们测试了直径约200 nm的纳米颗粒，与抗BPA抗体偶联，以增加免疫传感器表面的质量变化，从而增加检测到的频移。第二步，使用涂有抗BPA抗体的纳米颗粒，在BPA浓度低于10 ng mL（-1）时，将测定的灵敏度提高了约8倍。场发射扫描电子显微镜（FE-SEM）显示，聚合物2-甲基丙烯酰氧乙基磷酰胆碱（MPC）纳米粒子耦合到抗体保持单分散的免疫传感器的表面上，因此在免疫传感器中产生稳定的信号。由于在测定中检测到的频率偏移主要源自PZ晶体表面上的质量变化，因此在增强步骤中使用的抗体缀合颗粒的胶体稳定性在实现稳定且高灵敏度的信号方面起着极其重要的作用。
An immunoassay in which BPA competed with a BPA-horseradish peroxidase conjugate for binding to anti-BPA antibodies, coupled to a piezoelectric (PZ) immunosensor, was able to detect 0.1 ng mL(-1) BPA. To enhance the sensitivity of the assay, we tested nanoparticles approximately 200 nm in diameter, coupled to anti-BPA antibodies, to increase the mass change on the surface of the immunosensor and thereby increase the frequency shift detected. This second step, using nanoparticles coated with anti-BPA antibodies, improved the sensitivity of the assay by approximately eight times at BPA concentrations below 10 ng mL(-1). Field emission-scanning electron microscopy (FE-SEM) showed that polymeric 2-methacrolyloxyethyl phosphorylcholine (MPC) nanoparticles coupled to antibodies remained monodisperse on the surface of the immunosensor and therefore produced stable signals in the immunosensors. Since the frequency shift detected in the assay mainly originated from the mass change on the surface of the PZ crystal, the colloidal stability of the antibody-conjugated particles used in the enhancement step played an extremely important role in achieving a stable and highly sensitive signal.