Tissue distribution of P2X4 receptors studied with an ectodomain antibody
Tissue distribution of P2X4 receptors studied with an ectodomain antibody
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DOI：
10.1007/s00441-003-0758-5
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发表时间：
2003-01-01
影响因子：
3.6
通讯作者：
North, RA
中科院分区：
文献类型：
--
作者：
Bo, XN;Kim, M;North, RA
针对大鼠 P2X(4) 受体胞外结构域开发了单克隆抗体。该抗体在所有大鼠 P2X 受体亚基中具有高度选择性，并且仅识别 P2X(4) 受体的寡聚、非变性形式。免疫组织化学显示其广泛分布于中枢和周围神经系统、导管腺和气道上皮、膀胱平滑肌、胃肠道、子宫和动脉、子宫内膜和脂肪细胞。通过蛋白质印迹法在这些组织的膜提取物中鉴定出该蛋白质，并且胞外域抗体免疫沉淀了可被 P2X(4) 受体 C 末端抗体识别的蛋白质。研究结果表明，P2X(4) 受体亚基在哺乳动物组织中分布非常广泛，这表明可能具有新的功能作用。
A monoclonal antibody was developed to the extracellular domain of the rat P2X(4) receptor. The antibody was highly selective among all rat P2X receptor subunits, and recognised only the oligomeric, non-denatured form of the P2X(4) receptor. Immunohistochemistry showed an extensive pattern of distribution throughout the central and peripheral nervous systems, the epithelia of ducted glands and airways, smooth muscle of bladder, gastrointestinal tract, uterus, and arteries, uterine endometrium and fat cells. The protein was identified by Western blotting in membrane extracts of these tissues, and the ectodomain antibody immunoprecipitated a protein that was recognised with a P2X(4) receptor C terminus antibody. The findings indicate that the P2X(4) receptor subunit has a very extensive distribution among mammalian tissues, and this suggests possible new functional roles.