Rous sarcoma virus nucleic acid-binding protein p12 is necessary for viral 70S RNA dimer formation and packaging
Rous sarcoma virus nucleic acid-binding protein p12 is necessary for viral 70S RNA dimer formation and packaging
复制标题
劳斯肉瘤病毒核酸结合蛋白p12对于病毒70S RNA二聚体的形成和包装是必需的
作者：
C. Méric;P. Spahr
为了研究劳斯肉瘤病毒核酸结合蛋白p12的功能，我们通过使用布拉格C株的p12原病毒编码序列中的两个限制性位点插入KpnI合成接头来构建突变体。这两个限制性位点位于同一阅读框中，这使我们能够构建缺乏两个保守的 Cys-His 区域的缺失突变体和包含三个完整 Cys-His 框的重复突变体。这些突变 DNA 被转染到鸡胚成纤维细胞中，并对瞬时测定中产生的病毒颗粒进行生化和感染性表征。我们的结果表明，劳斯肉瘤病毒核酸结合蛋白 p12 对于基因组 RNA 包装是必需的，但对于颗粒组装不是必需的，并且与稳定的 70S 二聚体 RNA 的形成有关。此外，一种突变体显然能够包装正常的 70S RNA 但不具有感染性，这一事实表明 p12 在感染过程中发挥着作用。
To study the function(s) of the Rous sarcoma virus nucleic acid-binding protein p12, we constructed mutants by using two restriction sites in the p12 proviral coding sequence of the Prague C strain to insert KpnI synthetic linkers. The two restriction sites are in the same reading frame, which allowed us to construct a deletion mutant lacking the two conserved Cys-His regions and a duplication mutant containing three intact Cys-His boxes. These mutant DNAs were transfected into chicken embryo fibroblasts, and the viral particles produced in a transient assay were characterized biochemically and for infectivity. Our results indicate that the Rous sarcoma virus nucleic acid-binding protein p12 is necessary for genomic RNA packaging but not for particle assembly and is implicated in the formation of a stable 70S dimeric RNA. Moreover, the fact that one mutant was apparently able to package normal 70S RNA but was not infectious suggests a role for p12 during the infection process.
禽逆转录病毒核衣壳蛋白 pp12 磷酸化对病毒 RNA 结合的影响。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Leis,J;Johnson,S;Collins,LS;Traugh,JA
通讯作者：
Traugh,JA