The Costs and Benefits of Two Secondary Symbionts in a Whitefly Host Shape Their Differential Prevalence in the Field.
The Costs and Benefits of Two Secondary Symbionts in a Whitefly Host Shape Their Differential Prevalence in the Field.
复制标题
粉虱宿主中两种次生共生体的成本和效益决定了它们在田间的不同流行程度
DOI：
10.3389/fmicb.2021.739521
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发表时间：
2021
影响因子：
5.2
通讯作者：
Liu SS
中科院分区：
文献类型：
--
作者：
Shan HW;Liu SS
昆虫在自然界中普遍存在母系遗传的胞内共生体，而微生物伙伴往往会影响寄主的繁殖和适合性，从而促进它们的流行。在这里，我们调查了烟粉虱复合体的入侵种烟粉虱中共生菌的组成及其生物学效应。野外调查表明，MED粉虱种群除主要共生体Portiera外，主要含有两种次生共生体：哈密尔顿菌(Hamiltonella)，几乎固定在寄主种群中；Carddium，侵染率为0~86%。我们从一个田间种群中分离和建立了具有相似核遗传背景的Carddium阳性和无Carddium粉虱品系，并比较了这两个品系的性能。粉虱Carddium的感染对MED粉虱造成了巨大的适合度损失，包括生殖力和卵存活率的降低以及发育的延迟。然后，我们有选择地从不含Carddium的粉虱品系中移除汉密尔顿菌，并比较了两种粉虱品系的性能，其中一种同时含有Portiera和Hamiltonella，另一种只含有Portiera。虽然哈密尔顿氏菌的缺乏对白粉虱的繁殖力、发育率和后代存活率几乎没有影响，但无哈密尔顿氏菌的粉虱品系产生的雌性后代很少，通常会使后代雌性比从50%左右降到1%以下。我们的发现表明，这两种共生菌与MED粉虱之间的不同成本和收益可能在形成它们在该领域的不同流行率中发挥重要作用。
Insects commonly harbor maternally inherited intracellular symbionts in nature, and the microbial partners often exert influence on host reproduction and fitness to promote their prevalence. Here, we investigated composition of symbionts and their biological effects in the invasive Bemisia tabaci MED species of a whitefly complex. Our field surveys revealed that populations of the MED whitefly, in addition to the primary symbiont Portiera, mainly contain two secondary symbionts Hamiltonella, which is nearly fixed in the host populations, and Cardinium with infection frequencies ranging from 0 to 86%. We isolated and established Cardinium-positive and Cardinium-free whitefly lines with a similar nuclear genetic background from a field population, and compared performance of the two whitefly lines. The infection of Cardinium incurred significant fitness costs on the MED whitefly, including reduction of fecundity and egg viability as well as delay in development. We then selectively removed Hamiltonella from the Cardinium-free whitefly line and compared performance of two whitefly lines, one harboring both Portiera and Hamiltonella and the other harboring only Portiera. While depletion of Hamiltonella had little or only marginal effects on the fecundity, developmental rate, and offspring survival, the Hamiltonella-free whitefly line produced very few female offspring, often reducing the progeny female ratio from about 50% to less than 1%. Our findings indicate that the varying costs and benefits of the association between these two symbionts and the MED whitefly may play an important role in shaping their differential prevalence in the field.