A new caffeic acid ester and a new ceramide from the roots of Eriosema glomeratum
A new caffeic acid ester and a new ceramide from the roots of Eriosema glomeratum
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DOI：
10.1016/j.phytol.2021.07.019
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发表时间：
2021-10
影响因子：
1.7
通讯作者：
Gaetan T Tabakam;T. Kodama;A. R. N. Donfack;Y. M. M. Nguekeu-Y.-M.-M.-Nguekeu-8237747;Battsengel Nomin-Erdene;Zin Paing Htoo;K. Do;S. Ngouela;Mathieu Tene;H. Morita;M. Awouafack
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作者：
Gaetan T Tabakam;T. Kodama;A. R. N. Donfack;Y. M. M. Nguekeu-Y.-M.-M.-Nguekeu-8237747;Battsengel Nomin-Erdene;Zin Paing Htoo;K. Do;S. Ngouela;Mathieu Tene;H. Morita;M. Awouafack
通过对鹅掌菇CH2Cl2/MeOH(1：1，v/v)提取物的植物化学研究，得到一个新的咖啡酸酯(1)和一个新的神经酰胺(2)，以及8个已知化合物：染料木素(3)、染料木素(4)、羽扇豆异黄酮A(5)、槲皮素(6)、羽扇豆醇(7)、β-谷甾醇(8)和豆甾醇(9)、β-谷甾醇-3-O-β-d-吡喃葡萄糖苷(10)。通过对化合物的1D和2D-核磁共振、HRESI-MS、ESI-MS/MS等波谱数据的分析和化学转化，确定了新化合物的结构。测定了化合物1-−-6对人肺癌(A549)、乳腺癌细胞(MCF7)和宫颈癌细胞(HeLa)的细胞毒作用。化合物3和6对MCF7(IC_(50)=88.9和42.6μM)、A549(IC_(50)=42.1和28.2μM)和HeLa(IC_(50)=27.4和19.1μM)均有抑制作用。化合物4对HeLa癌细胞具有选择性中等活性，IC50值为68.7μM。
A new caffeic acid ester, eriocaffeate (1), and a new ceramide, erioceramide (2) along with eight known compounds, genistein (3), genistin (4), lupinisoflavone A (5), quercetin (6), lupeol (7), a mixture ofβ-sitosterol (8) and stigmasterol (9), andβ-sitosterol-3-O-β-d-glucopyranoside (10) were obtained after the phytochemical investigation of the CH2Cl2/MeOH (1:1, v/v) extract from the roots ofEriosema glomeratum. The structures of the new compounds were elucidated by analyses of their spectroscopic data including 1D- and 2D-NMR, HRESI-MS, and ESI-MS/MS as well as chemical conversions. The cytotoxicity of isolated compounds1−6was determined against three human [lung (A549), breast (MCF7), and cervical (HeLa)] cancer cell lines. Compounds3and6possessed the activities on all the tested three cell lines, MCF7 (IC50= 88.9 and 42.6 μM), A549 (IC50= 42.1 and 28.2 μM), and HeLa (IC50= 27.4 and 19.1 μM), respectively. Compound4showed selective moderate activity with the IC50value of 68.7 μM on the HeLa cancer cell line.