The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia
The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia
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DOI：
10.1152/ajplung.00256.2010
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发表时间：
2011-01-01
影响因子：
4.9
通讯作者：
Zabner, Joseph
中科院分区：
文献类型：
--
作者：
Pezzulo, Alejandro A.;Starner, Timothy D.;Zabner, Joseph
Pezzulo AA，Starner TD，Scheetz TE，Traver GL，蒂利AE，Harvey BG，Crystal RG，McCray PB Jr，Zabner J.空气-液体界面和原代细胞培养物的使用对于概括体内气道上皮细胞的转录谱是重要的。美国生理学杂志肺细胞分子生理学300：L25-L31，2011年。首次发表于2010年10月22日; doi：10.1152/ajplung.00256.2010.-原代人气道上皮细胞的器官型培养已被用于研究这种复杂组织的形态、离子和液体转运、先天免疫、转胞吞、感染、炎症、信号传导、纤毛和修复功能。然而，我们不知道这些培养物与体内气道表面上皮有多相似。在这项研究中，我们研究了气管和支气管的人气道上皮细胞在体内的全基因组表达谱，并将其与在气液界面生长的人气道上皮细胞的原代培养物的表达谱进行了比较。为了比较，我们还研究了在气液界面生长的Calu-3细胞和浸没在营养培养基中的人气道上皮的原代培养物的表达谱。我们发现，在气液界面生长的分化的原代培养物的转录谱最接近于体内气道上皮的转录谱，这表明使用原代培养物和存在气液界面对于概括气道上皮生物学是重要的。我们描述了不同人供体内和之间的气管和支气管来源的细胞之间的高度相似性，这表明气道细胞特异性的非常稳健的表达谱。
Pezzulo AA, Starner TD, Scheetz TE, Traver GL, Tilley AE, Harvey BG, Crystal RG, McCray PB Jr, Zabner J. The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia. Am J Physiol Lung Cell Mol Physiol 300: L25-L31, 2011. First published October 22, 2010; doi:10.1152/ajplung.00256.2010.-Organotypic cultures of primary human airway epithelial cells have been used to investigate the morphology, ion and fluid transport, innate immunity, transcytosis, infection, inflammation, signaling, cilia, and repair functions of this complex tissue. However, we do not know how closely these cultures resemble the airway surface epithelium in vivo. In this study, we examined the genome-wide expression profile of tracheal and bronchial human airway epithelia in vivo and compared it with the expression profile of primary cultures of human airway epithelia grown at the air-liquid interface. For comparison, we also investigated the expression profile of Calu-3 cells grown at the air-liquid interface and primary cultures of human airway epithelia submerged in nutrient media. We found that the transcriptional profile of differentiated primary cultures grown at the air-liquid interface most closely resembles that of in vivo airway epithelia, suggesting that the use of primary cultures and the presence of an air-liquid interface are important to recapitulate airway epithelia biology. We describe a high level of similarity between cells of tracheal and bronchial origin within and between different human donors, which suggests a very robust expression profile that is specific to airway cells.