Production and activation of recombinant papain-like cysteine proteases
Production and activation of recombinant papain-like cysteine proteases
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DOI：
10.1016/s1046-2023(03)00212-3
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发表时间：
2004-02-01
期刊：
影响因子：
4.8
通讯作者：
Turk, B
中科院分区：
文献类型：
--
作者：
Brömme, D;Nallaseth, FS;Turk, B
木瓜蛋白酶样半胱氨酸蛋白酶是半胱氨酸蛋白酶类中数量最多的家族。它们在整个动物和植物界以及病毒和细菌中表达。最近，这种蛋白酶家族作为以过度细胞外基质降解为特征的疾病（例如骨质疏松症、关节炎、血管疾病和癌症）中的潜在药物靶标引起了关注。此外，木瓜蛋白酶样半胱氨酸蛋白酶已被确定为各种人类和牲畜病原体以及主要过敏原的生命周期和侵入潜力的关键组分。因此，这类蛋白酶被严格研究，需要足够量的蛋白酶蛋白来分析结构-活性关系，它们的3-D结构，以及筛选和优化有效的选择性抑制剂。本文综述了通过在各种表达系统中异源表达产生活性木瓜蛋白酶样半胱氨酸蛋白酶的方法。(C)2003年爱思唯尔公司All rights reserved.
Papain-like cysteine proteases are the most numerous family of the cysteine protease class. They are expressed throughout the animal and plant kingdoms as well as in viruses and bacteria. More recently, this protease family has drawn attention as a potential pharmaceutical drug target in diseases characterized by excessive extracellular matrix degradation such as in osteoporosis, arthritis, vascular diseases, and cancer. Moreover, papain-like cysteine proteases have been identified as critical components of the life cycle and invasive potential of various human and live stock pathogens as well as major allergens. Therefore, this protease class is rigorously studied and requires sufficient amounts of protease protein to analyze structure-activity relationships, their 3-D structures as well as to screen for and optimize potent and selective inhibitors. This review summarizes approaches to generate active papain-like cysteine proteases by heterologous expression in a variety of expression systems. (C) 2003 Elsevier Inc. All rights reserved.